| RRC ID |
10852
|
| 著者 |
Inagaki Y, Hisatomi Y, Suzuki T, Kasahara K, Iida S.
|
| タイトル |
Isolation of a Suppressor-mutator/Enhancer-like transposable element, Tpn1, from Japanese morning glory bearing variegated flowers.
|
| ジャーナル |
Plant Cell
|
| Abstract |
The Japanese morning glory has an extensive history of genetic studies. Many mutants in the colors and shapes of its flowers and leaves have been isolated since the 17th century, and more than 200 genetic loci have been localized for the 10 linkage groups. They include over 20 mutable loci, several with variegated flower phenotypes. In a line of Japanese morning glory bearing variegated flowers called flecked, a transposable element of 6.4 kb, termed Tpn1, was found within one of the anthocyanin biosynthesis genes encoding dihydroflavonol-4-reductase (DFR). The 6.4-kb element carries 28-bp perfect terminal inverted repeats, the outer 13 bp being identical to those of the maize transposable element Suppressor-mutator/Enhancer. It is flanked by 3-bp direct repeats within the second intron of the DFR gene, 9 bp upstream of the third exon. When somatic and germinal excision occurs, it produces excision sequences characteristic of plant transposable elements. Cosegregation data of the variegated flower phenotype and the DFR gene carrying Tpn1 indicated that the mutable phenotype is due to excision of Tpn1 from the DFR gene. Sequences homologous to Tpn1 are present in multiple copies in the genome of Japanese morning glory.
|
| 巻・号 |
6(3)
|
| ページ |
375-83
|
| 公開日 |
1994-3-1
|
| DOI |
10.1105/tpc.6.3.375
|
| PII |
6/3/375
|
| PMID |
8180498
|
| PMC |
PMC160440
|
| MeSH |
Alcohol Oxidoreductases / genetics*
Base Sequence
Cloning, Molecular
Crosses, Genetic
DNA Transposable Elements / genetics*
Exons / genetics
Genomic Library
Germ Cells
Introns / genetics
Molecular Sequence Data
Mutagenesis
Plants / enzymology
Plants / genetics*
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid / genetics*
|
| IF |
9.618
|
| 引用数 |
89
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| アサガオ |
AK5
AK8
AK9 |
