| RRC ID |
10928
|
| Author |
Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.
|
| Title |
Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.
|
| Journal |
PLoS Genet
|
| Abstract |
Long interspersed elements (LINEs) are transposable elements that proliferate within eukaryotic genomes, having a large impact on eukaryotic genome evolution. LINEs mobilize via a process called retrotransposition. Although the role of the LINE-encoded protein(s) in retrotransposition has been extensively investigated, the participation of host-encoded factors in retrotransposition remains unclear. To address this issue, we examined retrotransposition frequencies of two structurally different LINEs--zebrafish ZfL2-2 and human L1--in knockout chicken DT40 cell lines deficient in genes involved in the non-homologous end-joining (NHEJ) repair of DNA and in human HeLa cells treated with a drug that inhibits NHEJ. Deficiencies of NHEJ proteins decreased retrotransposition frequencies of both LINEs in these cells, suggesting that NHEJ is involved in LINE retrotransposition. More precise characterization of ZfL2-2 insertions in DT40 cells permitted us to consider the possibility of dual roles for NHEJ in LINE retrotransposition, namely to ensure efficient integration of LINEs and to restrict their full-length formation.
|
| Volume |
5(4)
|
| Pages |
e1000461
|
| Published |
2009-4-1
|
| DOI |
10.1371/journal.pgen.1000461
|
| PMID |
19390601
|
| PMC |
PMC2666801
|
| MeSH |
Animals
Chickens
DNA Repair*
DNA Repair Enzymes / metabolism
HeLa Cells
Humans
Long Interspersed Nucleotide Elements*
Models, Genetic
Mutagenesis, Insertional*
Zebrafish
|
| IF |
5.175
|
| Times Cited |
73
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
DT40(RCB1464)
SHIP^(-) DT40(RCB1465)
IP3R^(-) DT40(RCB1467) |
