論文 - 詳細
| RRC ID | 10989 |
|---|---|
| 著者 | Pauloin T, Dutot M, Joly F, Warnet JM, Rat P. |
| タイトル | High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells. |
| ジャーナル | Mol Vis |
| Abstract |
PURPOSE:The aim of this study was to investigate high molecular weight hyaluronan (HMW-HA) protection on human corneal epithelial (HCE) cells against ultraviolet B (UVB) radiation-induced toxic effects. METHODS:The HCE cell line was incubated with HMW-HA or phosphate-buffered salt solution (PBS), rinsed, and exposed to UVB radiation. Cell viability, reactive oxygen species (ROS) and glutathione (GSH) levels, 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) release, p53 phosphorylation, caspase-3, -8, -9 activation, and interleukin (IL)-6 and -8 production were assessed to evaluate and to compare UVB-induced toxicity between cells treated with HMW-HA and cells treated with PBS. RESULTS:Data indicate that HMW-HA had significant protective effects against UVB radiation. HMW-HA increased HCE cell viability, decreased IL-6 and -8 production, and decreased caspase-3 and -8 activation. However, HMW-HA had no significant effect on ROS and GSH levels, 8-oxo-dG release, and p53 phosphorylation. CONCLUSIONS:To our knowledge, we report for the first time the ability of HMW-HA to protect cells against UV irradiation. According to our results, HMW-HA provides anti-inflammatory and anti-apoptotic signals to cells exposed to UVB. |
| 巻・号 | 15 |
| ページ | 577-83 |
| 公開日 | 2009-1-1 |
| PII | 58 |
| PMID | 19325714 |
| PMC | PMC2660376 |
| MeSH | 8-Hydroxy-2'-Deoxyguanosine Adjuvants, Immunologic / pharmacology Apoptosis* / drug effects Apoptosis* / radiation effects Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Line Cytoprotection / drug effects DNA Damage / drug effects Deoxyguanosine / analogs & derivatives Deoxyguanosine / metabolism Dose-Response Relationship, Radiation Epithelium, Corneal* / drug effects Epithelium, Corneal* / pathology Epithelium, Corneal* / radiation effects Glutathione / metabolism Humans Hyaluronic Acid / pharmacology* Inflammation Interleukin-6 / biosynthesis Interleukin-8 / biosynthesis Oxidative Stress / drug effects Oxidative Stress / radiation effects Reactive Oxygen Species / metabolism Tumor Suppressor Protein p53 / metabolism Ultraviolet Rays* |
| IF | 2.202 |
| 引用数 | 88 |
| WOS 分野 | OPHTHALMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |