| RRC ID |
11014
|
| Author |
Fierro-González JC, Cornils A, Alcedo J, Miranda-Vizuete A, Swoboda P.
|
| Title |
The thioredoxin TRX-1 modulates the function of the insulin-like neuropeptide DAF-28 during dauer formation in Caenorhabditis elegans.
|
| Journal |
PLoS One
|
| Abstract |
Thioredoxins comprise a conserved family of redox regulators involved in many biological processes, including stress resistance and aging. We report that the C. elegans thioredoxin TRX-1 acts in ASJ head sensory neurons as a novel modulator of the insulin-like neuropeptide DAF-28 during dauer formation. We show that increased formation of stress-resistant, long-lived dauer larvae in mutants for the gene encoding the insulin-like neuropeptide DAF-28 requires TRX-1 acting in ASJ neurons, upstream of the insulin-like receptor DAF-2. Genetic rescue experiments demonstrate that redox-independent functions of TRX-1 specifically in ASJ neurons are needed for the dauer formation constitutive (Daf-c) phenotype of daf-28 mutants. GFP reporters of trx-1 and daf-28 show opposing expression patterns in dauers (i.e. trx-1 is up-regulated and daf-28 is down-regulated), an effect that is not observed in growing L2/L3 larvae. In addition, functional TRX-1 is required for the down-regulation of a GFP reporter of daf-28 during dauer formation, a process that is likely subject to DAF-28-mediated feedback regulation. Our findings demonstrate that TRX-1 modulates DAF-28 signaling by contributing to the down-regulation of daf-28 expression during dauer formation. We propose that TRX-1 acts as a fluctuating neuronal signaling modulator within ASJ neurons to monitor the adjustment of neuropeptide expression, including insulin-like proteins, during dauer formation in response to adverse environmental conditions.
|
| Volume |
6(1)
|
| Pages |
e16561
|
| Published |
2011-1-27
|
| DOI |
10.1371/journal.pone.0016561
|
| PMID |
21304598
|
| PMC |
PMC3029385
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Environment
Gene Expression Regulation
Gene Expression Regulation, Developmental
Insulins
Larva / genetics
Larva / metabolism
Mutation
Neurons / physiology
Neuropeptides / physiology
Receptor, Insulin / genetics
Receptor, Insulin / metabolism*
Thioredoxins / genetics
Thioredoxins / metabolism*
|
| IF |
2.74
|
| Times Cited |
14
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| C.elegans |
tm2308 |
