論文 - 詳細
| RRC ID | 11067 |
|---|---|
| 著者 | Mattie FJ, Stackpole MM, Stone MC, Clippard JR, Rudnick DA, Qiu Y, Tao J, Allender DL, Parmar M, Rolls MM. |
| タイトル | Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:in many differentiated cells, microtubules are organized into polarized noncentrosomal arrays, yet few mechanisms that control these arrays have been identified. For example, mechanisms that maintain microtubule polarity in the face of constant remodeling by dynamic instability are not known. Drosophila neurons contain uniform-polarity minus-end-out microtubules in dendrites, which are often highly branched. Because undirected microtubule growth through dendrite branch points jeopardizes uniform microtubule polarity, we have used this system to understand how cells can maintain dynamic arrays of polarized microtubules. RESULTS:we find that growing microtubules navigate dendrite branch points by turning the same way, toward the cell body, 98% of the time and that growing microtubules track along stable microtubules toward their plus ends. Using RNAi and genetic approaches, we show that kinesin-2, and the +TIPS EB1 and APC, are required for uniform dendrite microtubule polarity. Moreover, the protein-protein interactions and localization of Apc2-GFP and Apc-RFP to branch points suggests that these proteins work together at dendrite branches. The functional importance of this polarity mechanism is demonstrated by the failure of neurons with reduced kinesin-2 to regenerate an axon from a dendrite. CONCLUSIONS:we conclude that microtubule growth is directed at dendrite branch points and that kinesin-2, APC, and EB1 are likely to play a role in this process. We propose that kinesin-2 is recruited to growing microtubules by +TIPS and that the motor protein steers growing microtubules at branch points. This represents a newly discovered mechanism for maintaining polarized arrays of microtubules. |
| 巻・号 | 20(24) |
| ページ | 2169-77 |
| 公開日 | 2010-12-21 |
| DOI | 10.1016/j.cub.2010.11.050 |
| PII | S0960-9822(10)01516-2 |
| PMID | 21145742 |
| PMC | PMC3035180 |
| MeSH | Animals Cell Polarity Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Dendrites / metabolism* Dendrites / ultrastructure Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / metabolism* Kinesins / genetics Kinesins / metabolism* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Microtubules / ultrastructure* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism |
| IF | 9.601 |
| 引用数 | 61 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 7293R-1 |