論文 - 詳細
| RRC ID | 11160 |
|---|---|
| 著者 | Gibert JM, Marcellini S, David JR, Schlötterer C, Simpson P. |
| タイトル | A major bristle QTL from a selected population of Drosophila uncovers the zinc-finger transcription factor poils-au-dos, a repressor of achaete-scute. |
| ジャーナル | Dev Biol |
| Abstract |
Traditional screens aiming at identifying genes regulating development have relied on mutagenesis. Here, we describe a new gene involved in bristle development, identified through the use of natural variation and selection. Drosophila melanogaster bears a pattern of 11 macrochaetes per heminotum. From a population initially sampled in Marrakech, a strain was selected for an increased number of thoracic macrochaetes. Using recombination and single nucleotide polymorphisms, the factor responsible was mapped to a single locus on the third chromosome, poils au dos, that encodes a zinc-finger-ZAD protein. The original, as well as new, presumed null, alleles of poils au dos, is associated with ectopic achaete-scute expression that results in the additional bristles. This suggests a possible role for Poils au dos as a repressor of achaete and scute. Ectopic expression appears to be independent of the activity of known cis-regulatory enhancer sequences at the achaete-scute complex that mediate activation at specific sites on the notum. The target sequences for Poils au dos activity were mapped to a 14 kb region around scute. In addition, we show that pad interacts synergistically with the repressor hairy and with Dpp signaling in posterior and anterior regions of the notum, respectively. |
| 巻・号 | 288(1) |
| ページ | 194-205 |
| 公開日 | 2005-12-1 |
| DOI | 10.1016/j.ydbio.2005.09.032 |
| PII | S0012-1606(05)00639-1 |
| PMID | 16216235 |
| MeSH | Alleles Amino Acid Sequence Animals Basic Helix-Loop-Helix Transcription Factors / antagonists & inhibitors* Basic Helix-Loop-Helix Transcription Factors / biosynthesis Basic Helix-Loop-Helix Transcription Factors / genetics DNA-Binding Proteins / antagonists & inhibitors* DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / physiology* Drosophila melanogaster / chemistry Drosophila melanogaster / genetics Drosophila melanogaster / physiology Gene Expression Regulation Molecular Sequence Data Mutation Phenotype Promoter Regions, Genetic Quantitative Trait Loci* Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / physiology* Sequence Alignment Transcription Factors / antagonists & inhibitors* Transcription Factors / biosynthesis Transcription Factors / genetics Transcription Factors / isolation & purification Transcription Factors / physiology Zinc Fingers / genetics Zinc Fingers / physiology* |
| IF | 2.896 |
| 引用数 | 12 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | NP line |