| RRC ID |
11200
|
| Author |
Rashid MH, Togo K, Ueda M, Miyamoto T.
|
| Title |
Characterization of bacteriocin produced by Streptococcus bovis J2 40-2 isolated from traditional fermented milk 'Dahi'.
|
| Journal |
Anim Sci J
|
| Abstract |
A bacteriocin-producing strain Streptococcus bovis J2 40-2 was isolated from traditional fermented milk 'Dahi' in Bangladesh. Despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as Listeria monocytogenes. Bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide pH range of 2.0-10.0. It was stable when heated to 110 degrees C for 20 min, but lost 25% of its activity when heated to 121 degrees C for 15 or 20 min. Optimum bacteriocin production (5600 AU/mL) was achieved when the strain was cultured at 37 degrees C for 24 h in MRS medium rather than in TYLG, GM17, or skim milk medium. Bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut-off MW: 3500) and gel filtration chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that bacteriocin had a molecular weight of approximately 4.5 kDa.
|
| Volume |
80(1)
|
| Pages |
70-8
|
| Published |
2009-2-1
|
| DOI |
10.1111/j.1740-0929.2008.00596.x
|
| PII |
ASJ596
|
| PMID |
20163471
|
| MeSH |
Animals
Anti-Bacterial Agents / analysis
Bacteriocins / analysis
Bacteriocins / biosynthesis
Bacteriocins / chemistry*
Bangladesh
Cattle
Culture Techniques
Cultured Milk Products / chemistry*
Cultured Milk Products / microbiology
Female
Food Microbiology*
Food Preservation
Hot Temperature
Hydrogen-Ion Concentration
Listeria monocytogenes / drug effects
Peptide Hydrolases
Streptococcus bovis / genetics
Streptococcus bovis / isolation & purification
Streptococcus bovis / metabolism*
|
| IF |
1.399
|
| Times Cited |
8
|
|
WOS Category
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
|
| Resource |
| General Microbes |
JCM 1025
JCM 5885
JCM 5887
JCM 5889
JCM 5890 |
