RRC ID 11539
Author Gómez AR, López-Varea A, Molnar C, de la Calle-Mustienes E, Ruiz-Gómez M, Gómez-Skarmeta JL, de Celis JF.
Title Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3.
Journal Dev Dyn
Abstract The extracellular signal-regulated kinase (ERK) is a key transducer of the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) signaling pathways, and its function is required in multiple processes during animal development. The activity of ERK depends on the phosphorylation state of conserved threonine and tyrosine residues, and this state is regulated by different kinases and phosphatases. A family of phosphatases with specificity toward both threonine and tyrosine residues in ERK (dual-specificity phosphatases) play a conserved role in its dephosphorylation and consequent inactivation. Here, we characterize the function of the dual-specificity phosphatase MKP3 in Drosophila EGFR and Xenopus FGFR signaling. The function of MKP3 is required during Drosophila wing vein formation and Xenopus anteroposterior neural patterning. We find that the expression of the MKP3 gene is localized in places of high EGFR and FGFR signaling. Furthermore, this restricted expression depends on ERK function both in Drosophila and Xenopus, suggesting that MKP3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway.
Volume 232(3)
Pages 695-708
Published 2005-3
DOI 10.1002/dvdy.20227
PMID 15704110
MeSH Alleles Amino Acid Sequence Amino Acid Substitution Animals Biomarkers / metabolism Body Patterning Catalytic Domain Chromosome Mapping Drosophila / embryology* Embryo, Nonmammalian ErbB Receptors / metabolism Exons Feedback, Physiological Gene Expression Regulation, Developmental Introns Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors Mitogen-Activated Protein Kinase 3 / chemistry Mitogen-Activated Protein Kinase 3 / genetics Mitogen-Activated Protein Kinase 3 / metabolism* Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / metabolism* Molecular Sequence Data Phosphorylation Protein Structure, Tertiary Receptors, Fibroblast Growth Factor / metabolism Sequence Homology, Amino Acid Signal Transduction Threonine / chemistry Tyrosine / chemistry Veins / growth & development Wings, Animal / growth & development Xenopus / embryology* ras Proteins / metabolism*
IF 2.852
Times Cited 40