RRC ID 11561
著者 Sano Y, Akimaru H, Okamura T, Nagao T, Okada M, Ishii S.
タイトル Drosophila activating transcription factor-2 is involved in stress response via activation by p38, but not c-Jun NH(2)-terminal kinase.
ジャーナル Mol Biol Cell
Abstract Activating transcription factor (ATF)-2 is a member of the ATF/cAMP response element-binding protein family of transcription factors, and its trans-activating capacity is enhanced by stress-activated protein kinases such as c-Jun NH(2)-terminal kinase (JNK) and p38. However, little is known about the in vivo roles played by ATF-2. Here, we identified the Drosophila homologue of ATF-2 (dATF-2) consisting of 381 amino acids. In response to UV irradiation and osmotic stress, Drosophila p38 (dp38), but not JNK, phosphorylates dATF-2 and enhances dATF-2-dependent transcription. Consistent with this, injection of dATF-2 double-stranded RNA (dsRNA) into embryos did not induce the dorsal closure defects that are commonly observed in the Drosophila JNK mutant. Furthermore, expression of the dominant-negative dp38 enhanced the aberrant wing phenotype caused by expression of a dominant-negative dATF-2. Similar genetic interactions between dATF-2 and the dMEKK1-dp38 signaling pathway also were observed in the osmotic stress-induced lethality of embryos. Loss of dATF-2 in Drosophila S2 cells by using dsRNA abrogated the induction of 40% of the osmotic stress-induced genes, including multiple immune response-related genes. This indicates that dATF-2 is a major transcriptional factor in stress-induced transcription. Thus, dATF-2 is critical for the p38-mediated stress response.
巻・号 16(6)
ページ 2934-46
公開日 2005-6-1
DOI 10.1091/mbc.e04-11-1008
PII E04-11-1008
PMID 15788564
PMC PMC1142437
MeSH Amino Acid Sequence Animals Blotting, Western Cells, Cultured Conserved Sequence Dimerization Drosophila / cytology Drosophila / embryology* Drosophila / genetics Embryo, Nonmammalian Enzyme Activation Gene Expression Regulation, Developmental* Genes, Insect Genes, Reporter Glutathione Transferase / metabolism In Situ Hybridization JNK Mitogen-Activated Protein Kinases* Luciferases / metabolism Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Osmolar Concentration Phosphorylation Protein Structure, Tertiary RNA, Messenger / metabolism Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Transcriptional Activation p38 Mitogen-Activated Protein Kinases / metabolism*
IF 3.791
引用数 26
WOS 分野 CELL BIOLOGY
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