RRC ID 11587
Author Chanut-Delalande H, Jung AC, Lin L, Baer MM, Bilstein A, Cabernard C, Leptin M, Affolter M.
Title A genetic mosaic analysis with a repressible cell marker screen to identify genes involved in tracheal cell migration during Drosophila air sac morphogenesis.
Journal Genetics
Abstract Branching morphogenesis of the Drosophila tracheal system relies on the fibroblast growth factor receptor (FGFR) signaling pathway. The Drosophila FGF ligand Branchless (Bnl) and the FGFR Breathless (Btl/FGFR) are required for cell migration during the establishment of the interconnected network of tracheal tubes. However, due to an important maternal contribution of members of the FGFR pathway in the oocyte, a thorough genetic dissection of the role of components of the FGFR signaling cascade in tracheal cell migration is impossible in the embryo. To bypass this shortcoming, we studied tracheal cell migration in the dorsal air sac primordium, a structure that forms during late larval development. Using a mosaic analysis with a repressible cell marker (MARCM) clone approach in mosaic animals, combined with an ethyl methanesulfonate (EMS)-mutagenesis screen of the left arm of the second chromosome, we identified novel genes implicated in cell migration. We screened 1123 mutagenized lines and identified 47 lines displaying tracheal cell migration defects in the air sac primordium. Using complementation analyses based on lethality, mutations in 20 of these lines were genetically mapped to specific genomic areas. Three of the mutants were mapped to either the Mhc or the stam complementation groups. Further experiments confirmed that these genes are required for cell migration in the tracheal air sac primordium.
Volume 176(4)
Pages 2177-87
Published 2007-8
DOI 10.1534/genetics.107.073890
PII genetics.107.073890
PMID 17603108
PMC PMC1950623
MeSH Air Sacs / cytology Air Sacs / growth & development* Animals Base Sequence Cell Movement / genetics Crosses, Genetic DNA Primers / genetics Drosophila Proteins / genetics Drosophila Proteins / physiology Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development* Drosophila melanogaster / physiology Female Fibroblast Growth Factors / genetics Fibroblast Growth Factors / physiology Genes, Insect* Genetic Complementation Test Genetic Markers Larva / cytology Larva / growth & development Male Morphogenesis Mosaicism Mutagenesis Phenotype Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / physiology Receptors, Fibroblast Growth Factor / genetics Receptors, Fibroblast Growth Factor / physiology Signal Transduction Trachea / cytology Trachea / growth & development*
IF 3.564
Times Cited 14