Reference - Detail
|Author||Stramer B, Winfield M, Shaw T, Millard TH, Woolner S, Martin P.|
|Title||Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos.|
By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
|MeSH||Animals Drosophila / embryology* Drosophila / genetics Drosophila Proteins / physiology Embryo, Nonmammalian GATA Transcription Factors / physiology Gene Expression* Green Fluorescent Proteins / metabolism Hemocytes / physiology Homozygote Intracellular Signaling Peptides and Proteins / genetics* Intracellular Signaling Peptides and Proteins / metabolism Macrophages / physiology* Mutation Oligonucleotide Array Sequence Analysis Up-Regulation Wounds and Injuries / etiology Wounds and Injuries / genetics|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|
|Drosophila||GADD45 enhancer trap (DGRC#103594)|