RRC ID 11630
Author Stramer B, Winfield M, Shaw T, Millard TH, Woolner S, Martin P.
Title Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos.
Journal EMBO Rep.
Abstract By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
Volume 9(5)
Pages 465-71
Published 2008-5
DOI 10.1038/embor.2008.34
PII embor200834
PMID 18344972
PMC PMC2373367
MeSH Animals Drosophila / embryology* Drosophila / genetics Drosophila Proteins / physiology Embryo, Nonmammalian GATA Transcription Factors / physiology Gene Expression* Green Fluorescent Proteins / metabolism Hemocytes / physiology Homozygote Intracellular Signaling Peptides and Proteins / genetics* Intracellular Signaling Peptides and Proteins / metabolism Macrophages / physiology* Mutation Oligonucleotide Array Sequence Analysis Up-Regulation Wounds and Injuries / etiology Wounds and Injuries / genetics
IF 8.383
Times Cited 32
Drosophila GADD45 enhancer trap (DGRC#103594)