論文 - 詳細
| RRC ID | 11643 |
|---|---|
| 著者 | Saito K, Inagaki S, Mituyama T, Kawamura Y, Ono Y, Sakota E, Kotani H, Asai K, Siomi H, Siomi MC. |
| タイトル | A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. |
| ジャーナル | Nature |
| Abstract |
PIWI-interacting RNAs (piRNAs) silence retrotransposons in Drosophila germ lines by associating with the PIWI proteins Argonaute 3 (AGO3), Aubergine (Aub) and Piwi. piRNAs in Drosophila are produced from intergenic repetitive genes and piRNA clusters by two systems: the primary processing pathway and the amplification loop. The amplification loop occurs in a Dicer-independent, PIWI-Slicer-dependent manner. However, primary piRNA processing remains elusive. Here we analysed piRNA processing in a Drosophila ovarian somatic cell line where Piwi, but not Aub or AGO3, is expressed; thus, only the primary piRNAs exist. In addition to flamenco, a Piwi-specific piRNA cluster, traffic jam (tj), a large Maf gene, was determined as a new piRNA cluster. piRNAs arising from tj correspond to the untranslated regions of tj messenger RNA and are sense-oriented. piRNA loading on to Piwi may occur in the cytoplasm. zucchini, a gene encoding a putative cytoplasmic nuclease, is required for tj-derived piRNA production. In tj and piwi mutant ovaries, somatic cells fail to intermingle with germ cells and Fasciclin III is overexpressed. Loss of tj abolishes Piwi expression in gonadal somatic cells. Thus, in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing. |
| 巻・号 | 461(7268) |
| ページ | 1296-9 |
| 公開日 | 2009-10-29 |
| DOI | 10.1038/nature08501 |
| PII | nature08501 |
| PMID | 19812547 |
| MeSH | Animals Argonaute Proteins Cell Adhesion Molecules, Neuronal / metabolism Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Endoribonucleases / metabolism Female Genes, Insect / genetics Genetic Loci / genetics Maf Transcription Factors, Large / genetics Maf Transcription Factors, Large / metabolism* Male Ovary / cytology Ovary / metabolism Phenotype Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* RNA / biosynthesis RNA / genetics RNA / metabolism* RNA Interference RNA Processing, Post-Transcriptional RNA-Induced Silencing Complex / genetics RNA-Induced Silencing Complex / metabolism* Testis / cytology Testis / metabolism |
| IF | 42.779 |
| 引用数 | 280 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | Df(2L)E55 (DGRC#106822) |