RRC ID 11714
Author Orihara-Ono M, Toriya M, Nakao K, Okano H.
Title Downregulation of Notch mediates the seamless transition of individual Drosophila neuroepithelial progenitors into optic medullar neuroblasts during prolonged G1.
Journal Dev. Biol.
Abstract The first step in the development of the Drosophila optic medullar primordia is the expansion of symmetrically dividing neuroepithelial cells (NEs); this step is then followed by the appearance of asymmetrically dividing neuroblasts (NBs). However, the mechanisms responsible for the change from NEs to NBs remain unclear. Here, we performed detailed analyses demonstrating that individual NEs are converted into NBs. We also showed that this transition occurs during an elongated G1 phase. During this G1 phase, the morphological features and gene expressions of each columnar NE changed dynamically. Once the NE-to-NB transition was completed, the former NE changed its cell-cycling behavior, commencing asymmetric division. We also found that Notch signaling pathway was activated just before the transition and was rapidly downregulated. Furthermore, the clonal loss of the Notch wild copy in the NE region near the medial edge caused the ectopic accumulation of Delta, leading to the precocious onset of transition. Taken together, these findings indicate that the activation of Notch signaling during a finite window coordinates the proper timing of the NE-to-NB transition.
Volume 351(1)
Pages 163-75
Published 2011-3-1
DOI 10.1016/j.ydbio.2010.12.044
PII S0012-1606(11)00002-9
PMID 21215740
MeSH Animals Bromodeoxyuridine / metabolism Cell Differentiation Down-Regulation Drosophila / cytology Drosophila / growth & development* Drosophila Proteins / physiology* Eye / innervation* G1 Phase* Intracellular Signaling Peptides and Proteins Membrane Proteins / physiology Neural Stem Cells / cytology* Neuroepithelial Cells / cytology* Neurogenesis Receptors, Notch / physiology* Signal Transduction
IF 3.262
Times Cited 18
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Drosophila