論文 - 詳細
| RRC ID | 11782 |
|---|---|
| 著者 | Ichise H, Ichise T, Ohtani O, Yoshida N. |
| タイトル | Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice. |
| ジャーナル | Development |
| Abstract |
The lymphatic vasculature originates from the blood vasculature through a mechanism relying on Prox1 expression and VEGFC signalling, and is separated and kept separate from the blood vasculature in a Syk- and SLP76-dependent manner. However, the mechanism by which lymphatic vessels are separated from blood vessels is not known. To gain an understanding of the vascular partitioning, we searched for the affected gene in a spontaneous mouse mutant exhibiting blood-filled lymphatic vessels, and identified a null mutation of the Plcg2 gene, which encodes phospholipase Cgamma2 (PLCgamma2), by positional candidate cloning. The blood-lymph shunt observed in PLCgamma2-null mice was due to aberrant separation of blood and lymphatic vessels. A similar phenotype was observed in lethally irradiated wild-type mice reconstituted with PLCgamma2-null bone marrow cells. These findings indicate that PLCgamma2 plays an essential role in initiating and maintaining the separation of the blood and lymphatic vasculature. |
| 巻・号 | 136(2) |
| ページ | 191-5 |
| 公開日 | 2009-1-1 |
| DOI | 10.1242/dev.025353 |
| PII | dev.025353 |
| PMID | 19056831 |
| MeSH | Animals Base Sequence Blood Vessels / abnormalities Blood Vessels / embryology* Blood Vessels / enzymology* Bone Marrow Transplantation Cells, Cultured DNA Primers / genetics Endothelial Cells / enzymology Female Humans Lymphatic Vessels / abnormalities Lymphatic Vessels / embryology* Lymphatic Vessels / enzymology* Mice Mice, Knockout Mice, Mutant Strains Mice, Transgenic Phospholipase C gamma / deficiency Phospholipase C gamma / genetics Phospholipase C gamma / physiology* Polymorphism, Genetic Pregnancy Radiation Chimera |
| IF | 5.611 |
| 引用数 | 62 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01252 |