RRC ID 11938
Author Dodgson J, Brown W, Rosa CA, Armstrong J.
Title Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.
Journal Eukaryot Cell
Abstract The organization and control of polarized growth through the cell cycle of Schizosaccharomyces pombe, a single-celled eukaryote, have been studied extensively. We have investigated the changes in these processes when S. pombe differentiates to form multicellular invasive mycelia and have found striking alterations to the behavior of some of the key regulatory proteins. Cells at the tips of invading filaments are considerably more elongated than cells growing singly and grow at one pole only. The filament tip follows a strict direction of growth through multiple cell cycles. A group of proteins involved in the growth process and actin regulation, comprising Spo20, Bgs4, activated Cdc42, and Crn1, are all concentrated at the growing tip, unlike their distribution at both ends of single cells. In contrast, several proteins implicated in microtubule-dependent organization of growth, including Tea1, Tea4, Mod5, and Pom1, all show the opposite effect and are relatively depleted at the growing end and enriched at the nongrowing end, although Tea1 appears to continue to be delivered to both ends. A third group acting at different stages of the cell cycle, including Bud6, Rga4, and Mid1, localize similarly in filaments and single cells, while Nif1 shows a reciprocal localization to Pom1.
Volume 9(11)
Pages 1788-97
Published 2010-11-1
DOI 10.1128/EC.00084-10
PII EC.00084-10
PMID 20870879
PMC PMC2976291
MeSH Gene Deletion Genes, Fungal Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Mycelium / growth & development Mycelium / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / growth & development* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Species Specificity Time-Lapse Imaging
IF 2.992
Times Cited 9
DNA material S. pombe entry clone SpENT02G12 (SPW000956) SpENT18E01 (SPW007297)
Yeast FY14674(H06/B04)