RRC ID 11960
Author Hamlet MR, Yergeau DA, Kuliyev E, Takeda M, Taira M, Kawakami K, Mead PE.
Title Tol2 transposon-mediated transgenesis in Xenopus tropicalis.
Journal Genesis
Abstract The diploid frog Xenopus tropicalis is becoming a powerful developmental genetic model system. Sequencing of the X. tropicalis genome is nearing completion and several labs are embarking on mutagenesis screens. We are interested in developing insertional mutagenesis strategies in X. tropicalis. Transposon-mediated insertional mutagenesis, once used exclusively in plants and invertebrate systems, is now more widely applicable to vertebrates. The first step in developing transposons as tools for mutagenesis is to demonstrate that these mobile elements function efficiently in the target organism. Here, we show that the Medaka fish transposon, Tol2, is able to stably integrate into the X. tropicalis genome and will serve as a powerful tool for insertional mutagenesis strategies in the frog.
Volume 44(9)
Pages 438-45
Published 2006-9-1
DOI 10.1002/dvg.20234
PMID 16906529
MeSH Animals Base Sequence Chorionic Gonadotropin / pharmacology Cysteine / pharmacology DNA / chemistry DNA / genetics DNA Transposable Elements / genetics* Embryo, Nonmammalian / metabolism Female Gene Transfer Techniques* Green Fluorescent Proteins / metabolism Humans Microinjections Models, Genetic Molecular Sequence Data Mutagenesis, Insertional Oryzias / genetics Plasmids / administration & dosage Plasmids / genetics Promoter Regions, Genetic Sequence Analysis, DNA Transgenes Transposases / genetics Xenopus / classification Xenopus / embryology Xenopus / genetics*
IF 1.76
Times Cited 74
Clawed frogs / Newts