RRC ID 12075
Author Jontes JD, Emond MR, Smith SJ.
Title In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals.
Journal J. Neurosci.
Abstract N-cadherin is a prominent component of developing and mature synapses, yet very little is known about its trafficking within neurons. To investigate N-cadherin dynamics in developing axons, we used in vivo two-photon time-lapse microscopy of N-cadherin--green fluorescent protein (Ncad-GFP), which was expressed in Rohon-Beard neurons of the embryonic zebrafish spinal cord. Ncad-GFP was present as either stable accumulations or highly mobile transport packets. The mobile transport packets were of two types: tubulovesicular structures that moved preferentially in the anterograde direction and discrete-punctate structures that exhibited bidirectional movement. Stable puncta of Ncad-GFP accumulated in the wake of the growth cone with a time course. Colocalization of Ncad-GFP puncta with synaptic markers suggests that N-cadherin is a very early component of nascent synapses. Expression of deletion mutants revealed a potential role of the extracellular domain in appropriate N-cadherin trafficking and targeting. These results are the first to characterize the trafficking of a synaptic cell-adhesion molecule in developing axons in vivo. In addition, we have begun to investigate the cell biology of N-cadherin trafficking and targeting in the context of an intact vertebrate embryo.
Volume 24(41)
Pages 9027-34
Published 2004-10-13
DOI 10.1523/JNEUROSCI.5399-04.2004
PII 24/41/9027
PMID 15483121
MeSH Animals Cadherins / genetics* Cadherins / metabolism* Cells, Cultured Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Green Fluorescent Proteins / genetics Membrane Proteins / metabolism Microscopy / methods Mutagenesis, Site-Directed Neurons / cytology Neurons / metabolism* Presynaptic Terminals / metabolism* Protein Transport / physiology R-SNARE Proteins Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Deletion Spinal Cord / cytology Spinal Cord / embryology Spinal Cord / metabolism* Zebrafish
IF 5.971
Times Cited 60
WOS Category NEUROSCIENCES
Resource
Zebrafish islet-1 enhancer