| RRC ID |
12075
|
| 著者 |
Jontes JD, Emond MR, Smith SJ.
|
| タイトル |
In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals.
|
| ジャーナル |
J Neurosci
|
| Abstract |
N-cadherin is a prominent component of developing and mature synapses, yet very little is known about its trafficking within neurons. To investigate N-cadherin dynamics in developing axons, we used in vivo two-photon time-lapse microscopy of N-cadherin--green fluorescent protein (Ncad-GFP), which was expressed in Rohon-Beard neurons of the embryonic zebrafish spinal cord. Ncad-GFP was present as either stable accumulations or highly mobile transport packets. The mobile transport packets were of two types: tubulovesicular structures that moved preferentially in the anterograde direction and discrete-punctate structures that exhibited bidirectional movement. Stable puncta of Ncad-GFP accumulated in the wake of the growth cone with a time course. Colocalization of Ncad-GFP puncta with synaptic markers suggests that N-cadherin is a very early component of nascent synapses. Expression of deletion mutants revealed a potential role of the extracellular domain in appropriate N-cadherin trafficking and targeting. These results are the first to characterize the trafficking of a synaptic cell-adhesion molecule in developing axons in vivo. In addition, we have begun to investigate the cell biology of N-cadherin trafficking and targeting in the context of an intact vertebrate embryo.
|
| 巻・号 |
24(41)
|
| ページ |
9027-34
|
| 公開日 |
2004-10-13
|
| DOI |
10.1523/JNEUROSCI.5399-04.2004
|
| PII |
24/41/9027
|
| PMID |
15483121
|
| PMC |
PMC6730076
|
| MeSH |
Animals
Cadherins / genetics*
Cadherins / metabolism*
Cells, Cultured
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology
Embryo, Nonmammalian / metabolism
Green Fluorescent Proteins / genetics
Membrane Proteins / metabolism
Microscopy / methods
Mutagenesis, Site-Directed
Neurons / cytology
Neurons / metabolism*
Presynaptic Terminals / metabolism*
Protein Transport / physiology
R-SNARE Proteins
Rats
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Sequence Deletion
Spinal Cord / cytology
Spinal Cord / embryology
Spinal Cord / metabolism*
Zebrafish
|
| IF |
5.674
|
| 引用数 |
68
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ゼブラフィッシュ |
islet-1 enhancer |
