論文 - 詳細
| RRC ID | 12075 |
|---|---|
| 著者 | Jontes JD, Emond MR, Smith SJ. |
| タイトル | In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals. |
| ジャーナル | J Neurosci |
| Abstract |
N-cadherin is a prominent component of developing and mature synapses, yet very little is known about its trafficking within neurons. To investigate N-cadherin dynamics in developing axons, we used in vivo two-photon time-lapse microscopy of N-cadherin--green fluorescent protein (Ncad-GFP), which was expressed in Rohon-Beard neurons of the embryonic zebrafish spinal cord. Ncad-GFP was present as either stable accumulations or highly mobile transport packets. The mobile transport packets were of two types: tubulovesicular structures that moved preferentially in the anterograde direction and discrete-punctate structures that exhibited bidirectional movement. Stable puncta of Ncad-GFP accumulated in the wake of the growth cone with a time course. Colocalization of Ncad-GFP puncta with synaptic markers suggests that N-cadherin is a very early component of nascent synapses. Expression of deletion mutants revealed a potential role of the extracellular domain in appropriate N-cadherin trafficking and targeting. These results are the first to characterize the trafficking of a synaptic cell-adhesion molecule in developing axons in vivo. In addition, we have begun to investigate the cell biology of N-cadherin trafficking and targeting in the context of an intact vertebrate embryo. |
| 巻・号 | 24(41) |
| ページ | 9027-34 |
| 公開日 | 2004-10-13 |
| DOI | 10.1523/JNEUROSCI.5399-04.2004 |
| PII | 24/41/9027 |
| PMID | 15483121 |
| PMC | PMC6730076 |
| MeSH | Animals Cadherins / genetics* Cadherins / metabolism* Cells, Cultured Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Green Fluorescent Proteins / genetics Membrane Proteins / metabolism Microscopy / methods Mutagenesis, Site-Directed Neurons / cytology Neurons / metabolism* Presynaptic Terminals / metabolism* Protein Transport / physiology R-SNARE Proteins Rats Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sequence Deletion Spinal Cord / cytology Spinal Cord / embryology Spinal Cord / metabolism* Zebrafish |
| IF | 5.674 |
| 引用数 | 68 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ゼブラフィッシュ | islet-1 enhancer |