Reference - Detail
|Author||Hardy ME, Ross LV, Chien CB.|
|Title||Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron.|
Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression.
|MeSH||Animals Animals, Genetically Modified Embryo, Nonmammalian / metabolism* Gene Expression Regulation, Developmental* HSP70 Heat-Shock Proteins / genetics Heat-Shock Response* Hot Temperature* Intercellular Signaling Peptides and Proteins / genetics Nerve Tissue Proteins / genetics Zebrafish / embryology* Zebrafish / genetics|
|WOS Category||ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY|
|Zebrafish||rw015d (Tg (hsp70:slit2-GFP))|