RRC ID 12093
Author Hardy ME, Ross LV, Chien CB.
Title Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron.
Journal Dev. Dyn.
Abstract Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue-specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression.
Volume 236(11)
Pages 3071-6
Published 2007-11
DOI 10.1002/dvdy.21318
PMID 17907198
MeSH Animals Animals, Genetically Modified Embryo, Nonmammalian / metabolism* Gene Expression Regulation, Developmental* HSP70 Heat-Shock Proteins / genetics Heat-Shock Response* Hot Temperature* Intercellular Signaling Peptides and Proteins / genetics Nerve Tissue Proteins / genetics Zebrafish / embryology* Zebrafish / genetics
IF 2.507
Times Cited 23
Zebrafish rw015d (Tg (hsp70:slit2-GFP))