| RRC ID |
12115
|
| 著者 |
Amo R, Aizawa H, Takahoko M, Kobayashi M, Takahashi R, Aoki T, Okamoto H.
|
| タイトル |
Identification of the zebrafish ventral habenula as a homolog of the mammalian lateral habenula.
|
| ジャーナル |
J Neurosci
|
| Abstract |
The mammalian habenula consists of the medial and lateral habenulae. Recent behavioral and electrophysiological studies suggested that the lateral habenula plays a pivotal role in controlling motor and cognitive behaviors by influencing the activity of dopaminergic and serotonergic neurons. Despite the functional significance, manipulating neural activity in this pathway remains difficult because of the absence of a genetically accessible animal model such as zebrafish. To address the level of lateral habenula conservation in zebrafish, we applied the tract-tracing technique to GFP (green fluorescent protein)-expressing transgenic zebrafish to identify habenular neurons that project to the raphe nuclei, a major target of the mammalian lateral habenula. Axonal tracing in live and fixed fish showed projection of zebrafish ventral habenula axons to the ventral part of the median raphe, but not to the interpeduncular nucleus where the dorsal habenula projected. The ventral habenula expressed protocadherin 10a, a specific marker of the rat lateral habenula, whereas the dorsal habenula showed no such expression. Gene expression analyses revealed that the ventromedially positioned ventral habenula in the adult originated from the region of primordium lateral to the dorsal habenula during development. This suggested that zebrafish habenulae emerge during development with mediolateral orientation similar to that of the mammalian medial and lateral habenulae. These findings indicated that the lateral habenular pathways are evolutionarily conserved pathways and might control adaptive behaviors in vertebrates through the regulation of monoaminergic activities.
|
| 巻・号 |
30(4)
|
| ページ |
1566-74
|
| 公開日 |
2010-1-27
|
| DOI |
10.1523/JNEUROSCI.3690-09.2010
|
| PII |
30/4/1566
|
| PMID |
20107084
|
| PMC |
PMC6633804
|
| MeSH |
Adaptation, Physiological / physiology
Animals
Animals, Genetically Modified
Axons / metabolism
Axons / ultrastructure
Biogenic Monoamines / metabolism
Biological Evolution
Biomarkers
Body Patterning / genetics
Brain Stem / cytology
Brain Stem / metabolism
Cadherins / metabolism
Carbocyanines
Dopamine / metabolism
Gene Expression Regulation, Developmental / genetics
Habenula / cytology*
Habenula / embryology
Habenula / metabolism
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Neural Pathways / cytology*
Neural Pathways / metabolism
Neuroanatomical Tract-Tracing Techniques / methods
Protocadherins
Raphe Nuclei / cytology*
Raphe Nuclei / metabolism
Rats
Rats, Long-Evans
Serotonin / metabolism
Species Specificity
Zebrafish / anatomy & histology*
Zebrafish / physiology
Zebrafish Proteins / metabolism
|
| IF |
5.674
|
| 引用数 |
130
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ゼブラフィッシュ |
RIKEN WT
rw0110b(Tg (brn3a-hsp70:GFP )) |
