論文 - 詳細
| RRC ID | 12133 |
|---|---|
| 著者 | Asakawa K, Kawakami K. |
| タイトル | A transgenic zebrafish for monitoring in vivo microtubule structures. |
| ジャーナル | Dev Dyn |
| Abstract |
The microtubule (MT) cytoskeleton plays crucial roles in brain development by regulating the proliferation of neuronal progenitor cells, neuronal migration and axon guidance. Methods for monitoring MT in the intact brain, however, have been limited in vertebrates. Here, we report a transgenic zebrafish line for monitoring MT in vivo. This reporter line carries a transgene encoding the green fluorescent protein (GFP) -tagged tubulin gene linked to the upstream activating sequence (UAS), the recognition sequence of the yeast Gal4 transcriptional activator. By crossing this reporter line with appropriate transgenic Gal4 driver lines, we induced the GFP-tagged tubulin in various cell types from the embryonic stages to the adult stage. In larvae expressing the modified tubulin, individual MT filaments and other MT structures, including the mitotic spindles in proliferating neuronal progenitor cells, were clearly visualized. Therefore, the transgenic UAS reporter line should be useful for directly monitoring MTs in the intact brain. |
| 巻・号 | 239(10) |
| ページ | 2695-9 |
| 公開日 | 2010-10-1 |
| DOI | 10.1002/dvdy.22400 |
| PMID | 20737511 |
| MeSH | Animals Animals, Genetically Modified Blotting, Southern Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Histones / genetics Histones / metabolism Microscopy, Fluorescence Microtubules / metabolism* Polymerase Chain Reaction Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Rhombencephalon / metabolism Spindle Apparatus / metabolism Tubulin / metabolism Zebrafish |
| IF | 3.275 |
| 引用数 | 19 |
| WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | hspGGFF27A UASGtuba2A SAGFF(LF)73A |