RRC ID 12150
Author Tanaka S, Okuno Y, Numazawa S, Yamamoto T, Shioda S, Yoshida T.
Title Brain responses to acute withdrawal in phenobarbital-dependent rats.
Journal Eur. J. Pharmacol.
Abstract Heat shock proteins (HSP) such as HO-1 and HSP27 have been implicated as functioning in a protective manner against oxidative and physical stress. The objective of the current study was to determine the role of HSPs in drug-withdrawal stress induced in phenobarbital-dependent rats. Increased expression of HO-1 and HSP27 was observed in the hippocampus and the cerebral cortex of phenobarbital-withdrawn rats. Gene expression was measured by Northern and Western blot analyses and in situ hybridization. The induction of HO-1 mRNA was suppressed by the administration of the NMDA receptor antagonist, (+)-5-methyl-10,11-dihydro-5H-dibenzo (a,d) cyclohepten-5,10-imine (MK801). Despite significant upregulation of glutamatergic transmission, neuronal cell degeneration was not apparent. These findings suggest that the induction of HO-1 and HSP27 during withdrawal from phenobarbital dependence may play a role in protection against glutamate toxicity.
Volume 421(2)
Pages 101-8
Published 2001-6-8
PII S0014-2999(01)01017-2
PMID 11399265
MeSH Animals Anticonvulsants / adverse effects* Brain / cytology Brain / drug effects* Brain / metabolism Cell Survival / drug effects Dizocilpine Maleate / pharmacology Excitatory Amino Acid Antagonists / pharmacology Gene Expression Regulation / drug effects HSP27 Heat-Shock Proteins Heat-Shock Proteins* Heme Oxygenase (Decyclizing) / genetics Heme Oxygenase (Decyclizing) / metabolism Heme Oxygenase-1 Hippocampus / cytology Hippocampus / drug effects Hippocampus / metabolism In Situ Hybridization Male Neoplasm Proteins / genetics Neoplasm Proteins / metabolism Phenobarbital / adverse effects* RNA, Messenger / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Rats Rats, Wistar Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors Receptors, N-Methyl-D-Aspartate / physiology Substance Withdrawal Syndrome / metabolism Substance-Related Disorders
IF 3.04
Times Cited 3
DNA material pRHO1 Rat heme oxygenase cDNA (RDB01208)