RRC ID 12158
Author Hirashima Y, Ueno H, Karasawa K, Yokoyama K, Setaka M, Takaku A.
Title Transfection of the plasma-type platelet-activating factor acetylhydrolase gene attenuates glutamate-induced apoptosis in cultured rat cortical neurons.
Journal Brain Res
Abstract Using an adenoviral vector, we induced overexpression of the plasma type of platelet-activating factor acetylhydrolase in cultured rat neurons. Neurons overexpressing this enzyme showed a decrease in glutamate-induced injury, mainly, apparent as decreased apoptosis. Reduction of lipid peroxidation by this enzyme and protection of mitochondrial function were demonstrated, and these may be the basis of the resistance to glutamate-induced neuronal injury that we observed.
Volume 885(1)
Pages 128-32
Published 2000-12-1
DOI 10.1016/s0006-8993(00)02852-3
PII S0006-8993(00)02852-3
PMID 11121539
MeSH 1-Alkyl-2-acetylglycerophosphocholine Esterase Adenoviridae / genetics Animals Apoptosis / drug effects Apoptosis / physiology* Cells, Cultured Cerebral Cortex / cytology Genetic Vectors Glutamic Acid / pharmacology Guinea Pigs Lac Operon Malondialdehyde / metabolism Mitochondria / metabolism Neurons / cytology* Neurons / enzymology* Phospholipases A / genetics* Rats Transfection
IF 2.733
Times Cited 16
DNA material Ax1w1 (RDB01746) AxCANLacZ (RDB03211).