RRC ID 12199
Author Nakamura T, Mura T, Saito K, Ohsawa T, Akiyoshi H, Sato K.
Title Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats.
Journal Biochem Biophys Res Commun
Abstract Hepatocyte nuclear factor-3 (HNF-3) isoforms are key factors for regulation of gene expression and differentiation in hepatocytes. HNF-3gamma is abundantly expressed in the mature liver, but down-regulated in primary cultured hepatocytes, in which some other hepatic gene expressions are also decreased. In this study, the primary hepatocytes were infected with the recombinant adenovirus carrying HNF-3gamma gene (AxCAHNF3gamma), and this led to marked induction of the HNF-3gamma gene. As a result, the expressions of albumin, catalase, and ornithine transcarbamylase (OTC) genes were also recovered to significant levels in the AxCAHNF3gamma-infected hepatocytes. Moreover, hepatocyte proliferation stimulated by epidermal growth factor (EGF) and insulin was also inhibited by AxCAHNF3gamma infection. Our results demonstrate that the enforced expression of HNF-3gamma gene can lead to conservation of some original liver functions in the primary cultured hepatocytes accompanied by morphological differentiation and growth inhibition.
Volume 253(2)
Pages 352-7
Published 1998-12-18
DOI 10.1006/bbrc.1998.9797
PII S0006-291X(98)99797-2
PMID 9878541
MeSH Adenoviridae / genetics* Animals Cell Differentiation / genetics Cells, Cultured DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics* DNA-Binding Proteins / physiology Down-Regulation / genetics Gene Expression Regulation Gene Transfer Techniques* Hepatocyte Nuclear Factor 3-gamma Liver / cytology* Liver / metabolism Liver / physiology* Male Nuclear Proteins / biosynthesis Nuclear Proteins / genetics* Nuclear Proteins / physiology Rats Rats, Wistar Recombination, Genetic Transcription Factors / biosynthesis Transcription Factors / genetics* Transcription Factors / physiology
IF 2.985
Times Cited 13
DNA material AxCALacZ Adex1CAlacZ (RDB02726)