論文 - 詳細
| RRC ID | 12203 |
|---|---|
| 著者 | Miyazawa K, Mori A, Miyata H, Akahane M, Ajisawa Y, Okudaira H. |
| タイトル | Regulation of interleukin-1beta-induced interleukin-6 gene expression in human fibroblast-like synoviocytes by p38 mitogen-activated protein kinase. |
| ジャーナル | J Biol Chem |
| Abstract |
Involvement of p38 mitogen-activated protein (MAP) kinase in interleukin (IL)-6 gene expression of human fibroblast-like synoviocytes (FLSs) was assessed. p38 MAP kinase was constitutively expressed in human FLSs and activated in response to IL-1beta. A pyridinylimidazole compound, SB203580, inhibited p38 MAP kinase activity in vivo, since the activity of MAPKAP kinase-2 (a substrate of p38 MAP kinase) in IL-1beta-stimulated FLSs was totally suppressed by it. SB203580 concentration-dependently inhibited protein production and gene expression of IL-6 by human FLSs. The effect of SB203580 was dependent on de novo protein synthesis. SB203580 significantly reduced the stability of IL-6 mRNA without affecting the rate of IL-6 gene transcription. Here, we provide evidence that p38 MAP kinase is activated in response to IL-1beta in human FLSs and is involved in IL-6 synthesis by stabilizing IL-6 mRNA. |
| 巻・号 | 273(38) |
| ページ | 24832-8 |
| 公開日 | 1998-9-18 |
| DOI | 10.1074/jbc.273.38.24832 |
| PII | S0021-9258(19)60156-0 |
| PMID | 9733787 |
| MeSH | Anti-Inflammatory Agents / pharmacology Arthritis, Rheumatoid / genetics Arthritis, Rheumatoid / immunology Arthritis, Rheumatoid / pathology Calcium-Calmodulin-Dependent Protein Kinases / metabolism* Cell Nucleus / metabolism Cells, Cultured Cycloheximide / pharmacology Dactinomycin / pharmacology Dose-Response Relationship, Drug Fibroblasts / drug effects Fibroblasts / immunology Gene Expression Regulation / drug effects Gene Expression Regulation / immunology* Humans Imidazoles / pharmacology Interleukin-1 / pharmacology* Interleukin-1 / physiology Interleukin-6 / biosynthesis Interleukin-6 / genetics* Intracellular Signaling Peptides and Proteins Kinetics Mitogen-Activated Protein Kinases* NF-kappa B / metabolism Osteoarthritis / genetics Osteoarthritis / immunology Osteoarthritis / pathology Protein Serine-Threonine Kinases / metabolism Pyridines / pharmacology Recombinant Proteins / pharmacology Synovial Membrane / drug effects Synovial Membrane / immunology* Synovial Membrane / pathology p38 Mitogen-Activated Protein Kinases |
| IF | 4.238 |
| 引用数 | 195 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pGEMhIL-6 GT Human IL-6 genomic clone (RDB01211) |