Reference - Detail
| RRC ID | 12206 |
|---|---|
| Author | Yamashita T, Murakami T, Otani S, Kuwajima M, Shima K. |
| Title | Leptin receptor signal transduction: OBRa and OBRb of fa type. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
We report herein the characterization of activities of signal transduction for three types of leptin receptors (OBRs) from rats, the OBRa, OBRb, and OBRb with fa mutation (OBRb-fa), by measurement of the levels of tyrosine phosphorylation of STAT3 (signal transducers and activators of transcription 3) and MAPK (mitogen-activated protein kinase), which are induced by leptin stimulation of CHO cells stably expressing the OBR (CHO-OBRb, CHO-OBRa, or CHO-OBRb-fa cells). As the result of leptin stimulation, enhanced levels of tyrosine phosphorylation of STAT3 and MAPK were detected in CHO-OBRb cells. In CHO-OBRb-fa cells, enhancement levels for both were lower than those in CHO-OBRb cells. In CHO-OBRa cells, only the phosphorylation of MAPK was detected. These data suggest that these reduced signaling activities cause obesity in fa/fa rats and that OBRa, which has been generally thought to be inactive at signaling, actually transmits signals through the MAPK pathway. |
| Volume | 246(3) |
| Pages | 752-9 |
| Published | 1998-5-29 |
| DOI | 10.1006/bbrc.1998.8689 |
| PII | S0006-291X(98)98689-2 |
| PMID | 9618284 |
| MeSH | Animals CHO Cells Calcium-Calmodulin-Dependent Protein Kinases / metabolism Carrier Proteins / classification Carrier Proteins / genetics Carrier Proteins / metabolism* Cricetinae DNA-Binding Proteins / metabolism Enzyme Activation Gene Expression Regulation Leptin MAP Kinase Kinase 1 Mitogen-Activated Protein Kinase Kinases* Mutation Phosphorylation Protein Serine-Threonine Kinases / antagonists & inhibitors Protein-Tyrosine Kinases / antagonists & inhibitors Proteins / metabolism* Rats Receptors, Cell Surface* Receptors, Cytokine / classification Receptors, Cytokine / genetics Receptors, Cytokine / metabolism* Receptors, Leptin Recombinant Proteins / metabolism STAT3 Transcription Factor Signal Transduction Trans-Activators / metabolism Transcription, Genetic |
| IF | 2.985 |
| Times Cited | 128 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| DNA material | 465.20 Mouse JunB (RDB01298) |