RRC ID 12362
Author Nakamura-Kubo M, Hirata A, Shimoda C, Nakamura T.
Title The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis.
Journal Mol Biol Cell
Abstract Sporulation in fission yeast represents a unique mode of cell division in which a new cell is formed within the cytoplasm of a mother cell. This event is accompanied by formation of the forespore membrane (FSM), which becomes the plasma membrane of spores. At prophase II, the spindle pole body (SPB) forms an outer plaque, from which formation of the FSM is initiated. Several components of the SPB play an indispensable role in SPB modification, and therefore in sporulation. In this paper, we report the identification of a novel SPB component, Spo7, which has a pleckstrin homology (PH) domain. We found that Spo7 was essential for initiation of FSM assembly, but not for SPB modification. Spo7 directly bound to Meu14, a component of the leading edge of the FSM, and was essential for proper localization of Meu14. The PH domain of Spo7 had affinity for phosphatidylinositol 3-phosphate (PI3P). spo7 mutants lacking the PH domain showed aberrant spore morphology, similar to that of meu14 and phosphatidylinositol 3-kinase (pik3) mutants. Our study suggests that Spo7 coordinates formation of the leading edge and initiation of FSM assembly, thereby accomplishing accurate formation of the FSM.
Volume 22(18)
Pages 3442-55
Published 2011-9-1
DOI 10.1091/mbc.E11-02-0125
PII mbc.E11-02-0125
PMID 21775631
PMC PMC3172268
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Membrane / metabolism* Chromosomes, Fungal / metabolism Gene Knockout Techniques Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Meiosis Membrane Proteins / genetics Membrane Proteins / metabolism* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Morphogenesis* Phosphatidylinositol Phosphates / metabolism Protein Binding Protein Structure, Tertiary Qa-SNARE Proteins / genetics Qa-SNARE Proteins / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / genetics Schizosaccharomyces / growth & development* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spores / metabolism
IF 3.791
Times Cited 5
Yeast FY7456(MKW5) FY7051(JZ670) FY7476(AB4) FY7251(TN4) FY7816(TN29) FY12295(YN67)? FY12710(YN68) FY12318(YN90) FY12492(YN314)