RRC ID 12410
Author Fernandes H, Dechering K, van Someren E, Steeghs I, Apotheker M, Mentink A, van Blitterswijk C, de Boer J.
Title Effect of chordin-like 1 on MC3T3-E1 and human mesenchymal stem cells.
Journal Cells Tissues Organs (Print)
Abstract Since the discovery that bone morphogenetic proteins (BMPs) are able to induce ectopic bone formation, considerable effort has been devoted to apply it for bone regeneration. BMP activity needs to be temporally and spatially controlled and the organism has devised ways to achieve it. Here we show that the BMP inhibitor chordin-like 1 can interfere with BMP2 signalling thereby affecting the osteogenic differentiation of MC3T3-E1 cells. Besides its function as a BMP antagonist, chordin-like 1 enhanced the proliferation of human mesenchymal stem cells (hMSCs) in a BMP2-independent manner. When MC3T3-E1 cells were exposed to recombinant chordin-like 1 there was an inhibition of alkaline phosphatase (ALP) expression, whereas in the case of hMSCs no effect was observed. However, chordin-like 1 dose-dependently increased the proliferation of hMSCs. This effect is probably BMP2 independent because the chordin-like 1 concentration that stimulates proliferation does not interfere with BMP signalling monitored by a Smad-dependent reporter gene. Our data point towards a novel, BMP-independent role of chordin-like 1 in hMSC proliferation.
Volume 191(6)
Pages 443-52
Published 2010
DOI 10.1159/000281825
PII 000281825
PMID 20130390
MeSH Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism Bone Morphogenetic Protein 2 / pharmacology Cell Line Cell Proliferation / drug effects Cells, Cultured Eye Proteins / genetics Eye Proteins / metabolism* Eye Proteins / pharmacology* Flow Cytometry Humans Mesenchymal Stromal Cells / cytology Mesenchymal Stromal Cells / drug effects* Mesenchymal Stromal Cells / metabolism* Mice Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Nerve Tissue Proteins / pharmacology* Oligonucleotide Array Sequence Analysis Osteogenesis / drug effects Osteogenesis / genetics
IF 1.275
Times Cited 5
Human and Animal Cells MC3T3-E1 (RCB1126)