RRC ID 12465
著者 Tada K, Susumu H, Sakuno T, Watanabe Y.
タイトル Condensin association with histone H2A shapes mitotic chromosomes.
ジャーナル Nature
Abstract Chromosome structure is dynamically regulated during cell division, and this regulation is dependent, in part, on condensin. The localization of condensin at chromosome arms is crucial for chromosome partitioning during anaphase. Condensin is also enriched at kinetochores but its precise role and loading machinery remain unclear. Here we show that fission yeast (Schizosaccharomyces pombe) kinetochore proteins Pcs1 and Mde4--homologues of budding yeast (Saccharomyces cerevisiae) monopolin subunits and known to prevent merotelic kinetochore orientation--act as a condensin 'recruiter' at kinetochores, and that condensin itself may act to clamp microtubule binding sites during metaphase. In addition to the regional recruitment factors, overall condensin association with chromatin is governed by the chromosomal passenger kinase Aurora B. Aurora-B-dependent phosphorylation of condensin promotes its association with histone H2A and H2A.Z, which we identify as conserved chromatin 'receptors' of condensin. Condensin phosphorylation and its deposition onto chromosome arms reach a peak during anaphase, when Aurora B kinase relocates from centromeres to the spindle midzone, where the separating chromosome arms are positioned. Our results elucidate the molecular basis for the spatiotemporal regulation of mitotic chromosome architecture, which is crucial for chromosome partitioning.
巻・号 474(7352)
ページ 477-83
公開日 2011-6-1
DOI 10.1038/nature10179
PII nature10179
PMID 21633354
MeSH Adenosine Triphosphatases / metabolism* Aurora Kinase B Aurora Kinases Binding Sites Cell Cycle Proteins / metabolism Chromatin / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosomes, Fungal / metabolism* DNA-Binding Proteins / metabolism* HeLa Cells Histones / metabolism* Humans Kinetochores / metabolism Microtubules / metabolism Mitosis* Multiprotein Complexes / metabolism* Nuclear Proteins / metabolism Phosphorylation Protein Binding Protein Serine-Threonine Kinases / metabolism Protein Transport Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism cdc25 Phosphatases / genetics cdc25 Phosphatases / metabolism
IF 42.779
引用数 126
WOS 分野 CELL BIOLOGY
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