RRC ID 12514
Author Shigehisa A, Okuzaki D, Kasama T, Tohda H, Hirata A, Nojima H.
Title Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation.
Journal Mol. Biol. Cell
Abstract The meiosis-specific mug28(+) gene of Schizosaccharomyces pombe encodes a putative RNA-binding protein with three RNA recognition motifs (RRMs). Live observations of meiotic cells that express Mug28 tagged with green fluorescent protein (GFP) revealed that Mug28 is localized in the cytoplasm, and accumulates around the nucleus from metaphase I to anaphase II. Disruption of mug28(+) generated spores with low viability, due to the aberrant formation of the forespore membrane (FSM). Visualization of the FSM in living cells expressing GFP-tagged Psy1, an FSM protein, indicated that mug28Delta cells harbored abnormal FSMs that contained buds, and had a delayed disappearance of Meu14, a leading edge protein. Electron microscopic observation revealed that FSM formation was abnormal in mug28Delta cells, showing bifurcated spore walls that were thicker than the nonbifurcated spore walls of the wild type. Analysis of Mug28 mutants revealed that RRM3, in particular phenylalanin-466, is of primary importance for the proper localization of Mug28, spore viability, and FSM formation. Together, we conclude that Mug28 is essential for the proper maturation of the FSM and the spore wall.
Volume 21(12)
Pages 1955-67
Published 2010-6-15
DOI 10.1091/mbc.e09-12-0997
PII E09-12-0997
PMID 20410137
PMC PMC2883940
MeSH Amino Acid Motifs Amino Acid Substitution / genetics Cell Cycle Proteins / chemistry Cell Cycle Proteins / metabolism* Cell Membrane / metabolism Cell Membrane / ultrastructure Cell Wall / metabolism* Cell Wall / ultrastructure Cytoplasm / metabolism Green Fluorescent Proteins / metabolism Meiosis* Mutant Proteins / metabolism Phenotype Protein Transport RNA, Fungal / metabolism RNA-Binding Proteins / chemistry RNA-Binding Proteins / metabolism* Recombinant Fusion Proteins Schizosaccharomyces / cytology* Schizosaccharomyces / metabolism* Schizosaccharomyces / physiology Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / metabolism* Sequence Deletion / genetics Spores, Fungal / cytology Spores, Fungal / metabolism* Spores, Fungal / ultrastructure Subcellular Fractions / metabolism Time Factors
IF 3.512
Times Cited 3
Yeast (YN68 SOP091w AS117 AS160 AS161 JZ670 AS181 AS99 AS117 AS164 AS176) ?