論文 - 詳細
| RRC ID | 12526 |
|---|---|
| 著者 | Lightfoot J, Testori S, Barroso C, Martinez-Perez E. |
| タイトル | Loading of meiotic cohesin by SCC-2 is required for early processing of DSBs and for the DNA damage checkpoint. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:Chromosome segregation and the repair of DNA double-strand breaks (DSBs) by homologous recombination require cohesin, the protein complex that mediates sister chromatid cohesion (SCC). In addition, cohesin is also required for the integrity of DNA damage checkpoints in somatic cells, where cohesin loading depends on a conserved complex containing the Scc2/Nipbl protein. Although cohesin is required for the completion of meiotic recombination, little is known about how cohesin promotes the repair of meiotic DSBs and about the factors that promote loading of cohesin during meiosis. RESULTS:Here we show that during Caenorhabditis elegans meiosis, loading of cohesin requires SCC-2, whereas the cohesin-related complexes condensin and SMC-5/6 can be loaded by mechanisms independent of both SCC-2 and cohesin. Although the lack of cohesin in scc-2 mutants impairs the repair of meiotic DSBs, surprisingly, the persistent DNA damage fails to trigger an apoptotic response of the conserved pachytene DNA damage checkpoint. Mutants carrying an scc-3 allele that abrogates loading of meiotic cohesin are also deficient in the apoptotic response of the pachytene checkpoint, and both scc-2 and scc-3 mutants fail to recruit the DNA damage sensor 9-1-1 complex onto persistent damage sites during meiosis. Furthermore, we show that meiotic cohesin is also required for the timely loading of the RAD-51 recombinase to irradiation-induced DSBs. CONCLUSIONS:We propose that meiotic cohesin promotes DSB processing and recruitment of DNA damage checkpoint proteins, thus implicating cohesin in the earliest steps of the DNA damage response during meiosis. |
| 巻・号 | 21(17) |
| ページ | 1421-30 |
| 公開日 | 2011-9-13 |
| DOI | 10.1016/j.cub.2011.07.007 |
| PII | S0960-9822(11)00776-7 |
| PMID | 21856158 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism* Chromosome Segregation DNA Breaks, Double-Stranded* DNA Damage DNA Repair* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Homologous Recombination Meiosis* Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Pachytene Stage |
| IF | 9.601 |
| 引用数 | 32 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm2334 |