Reference - Detail
| RRC ID | 12542 |
|---|---|
| Author | Chen ES, Saitoh S, Yanagida M, Takahashi K. |
| Title | A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast. |
| Journal | Mol Cell |
| Abstract |
CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function. |
| Volume | 11(1) |
| Pages | 175-87 |
| Published | 2003-1-1 |
| DOI | 10.1016/s1097-2765(03)00011-x |
| PII | S1097-2765(03)00011-X |
| PMID | 12535531 |
| MeSH | Amino Acid Motifs Amino Acid Sequence Animals Cell Cycle / physiology* Centromere / metabolism* Chromosomal Proteins, Non-Histone / metabolism* Chromosomes / genetics Chromosomes / metabolism DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* GATA Transcription Factors Histones / metabolism Humans In Situ Hybridization, Fluorescence Molecular Sequence Data Phenotype Protein Binding Schizosaccharomyces / cytology Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Alignment |
| IF | 15.584 |
| Times Cited | 105 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Yeast | genomic library |