| RRC ID |
12572
|
| 著者 |
Rodríguez-Sánchez L, Rodríguez-López M, García Z, Tenorio-Gómez M, Schvartzman JB, Krimer DB, Hernández P.
|
| タイトル |
The fission yeast rDNA-binding protein Reb1 regulates G1 phase under nutritional stress.
|
| ジャーナル |
J Cell Sci
|
| Abstract |
Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found that Schizosaccharomyces pombe Reb1 also upregulates transcription of the ste9(+) gene that is required for nitrogen-starvation-induced growth arrest with a G1 DNA content and sexual differentiation. Ste9 activates the anaphase-promoting complex or cyclosome ('APC/C') in G1, targeting B-cyclin for proteasomal degradation in response to nutritional stress. Reb1 binds in vivo and in vitro to a specific DNA sequence at the promoter of ste9(+), similar to the sequence recognized in the rDNA, and this binding is required for ste9(+) transcriptional activation and G1 arrest. This suggests that Reb1 acts as a link between rDNA metabolism and cell cycle control in response to nutritional stress. In agreement with this new role for Reb1 in the regulation of the G1-S transition, reb1Δ and wee1(ts) mutations are synthetically lethal owing to the inability of these cells to lengthen G1 before entering S phase. Similarly, reb1Δ cdc10(ts) cells are unable to arrest in G1 and die at the semi-permissive temperature.
|
| 巻・号 |
124(Pt 1)
|
| ページ |
25-34
|
| 公開日 |
2011-1-1
|
| DOI |
10.1242/jcs.070987
|
| PII |
jcs.070987
|
| PMID |
21118960
|
| MeSH |
Base Sequence
DNA, Ribosomal / genetics
DNA, Ribosomal / metabolism*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
G1 Phase*
Gene Expression Regulation, Fungal*
Molecular Sequence Data
Nitrogen / deficiency
Protein Binding
Schizosaccharomyces / cytology
Schizosaccharomyces / genetics
Schizosaccharomyces / growth & development
Schizosaccharomyces / physiology*
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Stress, Physiological
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
4.573
|
| 引用数 |
16
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 酵母 |
|
