Reference - Detail
|Author||Fujita A, Misumi Y.|
|Title||Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.|
|Journal||Mol Biol Rep|
In fission yeast Schizosaccharomyces pombe, the directions of cell growth change from a monopolar manner to a bipolar manner, which is known as 'New End Take Off' (NETO). We previously found that Arf6, a member (class III) of the ADP-ribosylation factor GTPase (Arf) family, is necessary for NETO in fission yeast. Here we report the characterization of a S. pombe gene, ucp3, encoding a putative Arf GTPase-activating protein (GAP) for Arf6. The Ucp3 contains Arf GAP domain, and has a high similarity to Gts1, which was identified as a GAP for Arf3 (class III Arf) in Saccharomyces cerevisiae. Overexpression of ucp3 inhibited growth from new end possibly by disturbing the GDP/GTP-cycling of Arf6. Gene disruption of ucp3 revealed that Ucp3 is essential for cell viability. Ucp3 uniformly localizes to the cell periphery. And its localization is not dependent on microtubules, actin cytoskeletons, Arf6 and Syt22 (guanine nucleotide exchange factor for Arf6). We hypothesize that Ucp3 functions as a GAP for Arf6. Moreover, Ucp3 might have another function important for cell viability.
|MeSH||Actins / metabolism Amino Acid Sequence GTPase-Activating Proteins / chemistry GTPase-Activating Proteins / genetics* GTPase-Activating Proteins / metabolism Gene Deletion Genes, Fungal / genetics* Ion Channels / chemistry Ion Channels / genetics* Ion Channels / metabolism Microbial Viability Microtubules / metabolism Mitochondrial Proteins / chemistry Mitochondrial Proteins / genetics* Mitochondrial Proteins / metabolism Models, Biological Molecular Sequence Data Protein Transport Schizosaccharomyces / cytology Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Subcellular Fractions / metabolism Uncoupling Protein 3|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY|
|Yeast||TM214 FY12780 FY12785|