RRC ID 12615
Author Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Takaiwa F.
Title Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene.
Journal Theor Appl Genet
Abstract The rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased (alpha-1H/alpha-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6.50/alpha-1. Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele. Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6.50/alpha-1 of high homology with the deleted pI6.71/alpha-2 polypeptide which was encoded by glu4 (GluA-1). The nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene. These results suggested the possibility of altering glutelin quality by using single gene mutation.
Volume 107(1)
Pages 20-5
Published 2003-6-1
DOI 10.1007/s00122-003-1228-x
PMID 12835929
MeSH Alleles Amino Acid Sequence Base Sequence DNA, Plant / genetics Electrophoresis, Gel, Two-Dimensional Glutens / genetics* Glutens / metabolism Isoelectric Point Molecular Sequence Data Oryza / genetics* Oryza / metabolism Peptide Mapping Point Mutation Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
IF 4.439
Times Cited 7
Rice Induced Mutation Lines