論文 - 詳細
| RRC ID | 12652 |
|---|---|
| 著者 | Adachi H, Tsujimoto M. |
| タイトル | Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
The scavenger receptor FEEL-1/stabilin-1 is known as the marker of alternatively activated macrophage and sinusoidal endothelial cell. FEEL-1/stabilin-1 is a multifunctional transmembrane glycoprotein that is implicated in bacterial infection, diabetes, atherosclerosis, wound healing, and innate immunity. In the current study, we have identified the phox-homology domain containing protein SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells. SNX17 directly interacts with FEEL-1/stabilin-1 and regulates its trafficking. Studies using the cytoplasmic domain of truncated or mutant FEEL-1/stabilin-1 suggest that the NPxF motif of the FEEL-1/stabilin-1 cytoplasmic tail is required for its interaction with SNX17. By transfecting cells with small interfering RNA targeting SNX17, total cellular FEEL-1/stabilin-1 expression and FEEL-1/stabilin-1-mediated ligand uptake were significantly decreased due to the enhancement of FEEL-1/stabilin-1 protein degradation. Our results identify SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells. |
| 巻・号 | 1803(5) |
| ページ | 553-63 |
| 公開日 | 2010-5-1 |
| DOI | 10.1016/j.bbamcr.2010.02.011 |
| PII | S0167-4889(10)00069-8 |
| PMID | 20226821 |
| MeSH | Amino Acid Sequence Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / metabolism* Cell Membrane / metabolism* Cells, Cultured Endothelium, Vascular / cytology Endothelium, Vascular / metabolism* Gene Library Humans Molecular Sequence Data Protein Structure, Tertiary RNA, Small Interfering / pharmacology Receptors, Lymphocyte Homing / genetics Receptors, Lymphocyte Homing / metabolism* Sequence Homology, Amino Acid Sorting Nexins Two-Hybrid System Techniques Umbilical Veins / cytology Umbilical Veins / metabolism* Vesicular Transport Proteins / antagonists & inhibitors Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 3.411 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | FYP453(pTN184 (pBS-myc in XbaI(KS)) BY4121(EGY48) |