RRC ID 12652
著者 Adachi H, Tsujimoto M.
タイトル Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface.
ジャーナル Biochim Biophys Acta
Abstract The scavenger receptor FEEL-1/stabilin-1 is known as the marker of alternatively activated macrophage and sinusoidal endothelial cell. FEEL-1/stabilin-1 is a multifunctional transmembrane glycoprotein that is implicated in bacterial infection, diabetes, atherosclerosis, wound healing, and innate immunity. In the current study, we have identified the phox-homology domain containing protein SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells. SNX17 directly interacts with FEEL-1/stabilin-1 and regulates its trafficking. Studies using the cytoplasmic domain of truncated or mutant FEEL-1/stabilin-1 suggest that the NPxF motif of the FEEL-1/stabilin-1 cytoplasmic tail is required for its interaction with SNX17. By transfecting cells with small interfering RNA targeting SNX17, total cellular FEEL-1/stabilin-1 expression and FEEL-1/stabilin-1-mediated ligand uptake were significantly decreased due to the enhancement of FEEL-1/stabilin-1 protein degradation. Our results identify SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells.
巻・号 1803(5)
ページ 553-63
公開日 2010-5-1
DOI 10.1016/j.bbamcr.2010.02.011
PII S0167-4889(10)00069-8
PMID 20226821
MeSH Amino Acid Sequence Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / metabolism* Cell Membrane / metabolism* Cells, Cultured Endothelium, Vascular / cytology Endothelium, Vascular / metabolism* Gene Library Humans Molecular Sequence Data Protein Structure, Tertiary RNA, Small Interfering / pharmacology Receptors, Lymphocyte Homing / genetics Receptors, Lymphocyte Homing / metabolism* Sequence Homology, Amino Acid Sorting Nexins Two-Hybrid System Techniques Umbilical Veins / cytology Umbilical Veins / metabolism* Vesicular Transport Proteins / antagonists & inhibitors Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism*
IF 3.411
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 FYP453(pTN184 (pBS-myc in XbaI(KS)) BY4121(EGY48)