RRC ID |
12652
|
著者 |
Adachi H, Tsujimoto M.
|
タイトル |
Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface.
|
ジャーナル |
Biochim Biophys Acta
|
Abstract |
The scavenger receptor FEEL-1/stabilin-1 is known as the marker of alternatively activated macrophage and sinusoidal endothelial cell. FEEL-1/stabilin-1 is a multifunctional transmembrane glycoprotein that is implicated in bacterial infection, diabetes, atherosclerosis, wound healing, and innate immunity. In the current study, we have identified the phox-homology domain containing protein SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells. SNX17 directly interacts with FEEL-1/stabilin-1 and regulates its trafficking. Studies using the cytoplasmic domain of truncated or mutant FEEL-1/stabilin-1 suggest that the NPxF motif of the FEEL-1/stabilin-1 cytoplasmic tail is required for its interaction with SNX17. By transfecting cells with small interfering RNA targeting SNX17, total cellular FEEL-1/stabilin-1 expression and FEEL-1/stabilin-1-mediated ligand uptake were significantly decreased due to the enhancement of FEEL-1/stabilin-1 protein degradation. Our results identify SNX17 as a novel interaction partner of FEEL-1/stabilin-1 in endothelial cells.
|
巻・号 |
1803(5)
|
ページ |
553-63
|
公開日 |
2010-5-1
|
DOI |
10.1016/j.bbamcr.2010.02.011
|
PII |
S0167-4889(10)00069-8
|
PMID |
20226821
|
MeSH |
Amino Acid Sequence
Cell Adhesion Molecules, Neuronal / genetics
Cell Adhesion Molecules, Neuronal / metabolism*
Cell Membrane / metabolism*
Cells, Cultured
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism*
Gene Library
Humans
Molecular Sequence Data
Protein Structure, Tertiary
RNA, Small Interfering / pharmacology
Receptors, Lymphocyte Homing / genetics
Receptors, Lymphocyte Homing / metabolism*
Sequence Homology, Amino Acid
Sorting Nexins
Two-Hybrid System Techniques
Umbilical Veins / cytology
Umbilical Veins / metabolism*
Vesicular Transport Proteins / antagonists & inhibitors
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*
|
IF |
3.411
|
引用数 |
18
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
酵母 |
FYP453(pTN184 (pBS-myc in XbaI(KS))
BY4121(EGY48) |