RRC ID 12679
著者 Iwaki T, Osawa F, Onishi M, Koga T, Fujita Y, Hosomi A, Tanaka N, Fukui Y, Takegawa K.
タイトル Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe.
ジャーナル Yeast
Abstract From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33(+) gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33(+) gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells.
巻・号 20(10)
ページ 845-55
公開日 2003-7-30
DOI 10.1002/yea.1011
PMID 12868054
MeSH Amino Acid Sequence Blotting, Western Carboxypeptidases / genetics Carboxypeptidases / metabolism Cathepsin A Genes, Fungal / genetics Genes, Fungal / physiology* Genetic Complementation Test Microscopy, Fluorescence Molecular Sequence Data Mutagenesis, Insertional Protein Transport / genetics Protein Transport / physiology Pyridinium Compounds / metabolism Quaternary Ammonium Compounds / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid Vacuoles / genetics* Vacuoles / metabolism
IF 3.143
引用数 32
WOS 分野 MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 strains and plasmids