RRC ID 12726
Author Hirota Y, Meunier A, Huang S, Shimozawa T, Yamada O, Kida YS, Inoue M, Ito T, Kato H, Sakaguchi M, Sunabori T, Nakaya MA, Nonaka S, Ogura T, Higuchi H, Okano H, Spassky N, Sawamoto K.
Title Planar polarity of multiciliated ependymal cells involves the anterior migration of basal bodies regulated by non-muscle myosin II.
Journal Development
Abstract Motile cilia generate constant fluid flow over epithelial tissue, and thereby influence diverse physiological processes. Such functions of ciliated cells depend on the planar polarity of the cilia and on their basal bodies being oriented in the downstream direction of fluid flow. Recently, another type of basal body planar polarity, characterized by the anterior localization of the basal bodies in individual cells, was reported in the multiciliated ependymal cells that line the surface of brain ventricles. However, little is known about the cellular and molecular mechanisms by which this polarity is established. Here, we report in mice that basal bodies move in the apical cell membrane during differentiation to accumulate in the anterior region of ependymal cells. The planar cell polarity signaling pathway influences basal body orientation, but not their anterior migration, in the neonatal brain. Moreover, we show by pharmacological and genetic studies that non-muscle myosin II is a key regulator of this distribution of basal bodies. This study demonstrates that the orientation and distribution of basal bodies occur by distinct mechanisms.
Volume 137(18)
Pages 3037-46
Published 2010-9
DOI 10.1242/dev.050120
PII dev.050120
PMID 20685736
MeSH Animals Cell Differentiation Cell Movement* Cell Polarity* Cells, Cultured Cilia / metabolism Ependyma / cytology Ependyma / growth & development* Ependyma / metabolism* Gene Expression Regulation, Developmental Mice Mice, Inbred ICR Microscopy, Electron, Scanning Myosin Type II / genetics Myosin Type II / metabolism* Protein Biosynthesis
IF 5.413
Times Cited 50
WOS Category DEVELOPMENTAL BIOLOGY
Resource
DNA material CSII-EF-MCS (RDB04378)