| RRC ID |
12751
|
| Author |
Kanemura H, Satoh T, Bilasy SE, Ueda S, Hirashima M, Kataoka T.
|
| Title |
Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
RA-GEF-1 is a guanine nucleotide exchange factor for the small GTPase Rap1. RA-GEF-1 knockout mice show defects in vascular development starting around 7.5days post coitum and die by 9.5days post coitum. Here, we employed in vitro culture systems for allantois explants and endothelial cells to gain insights into the mechanism for RA-GEF-1-mediated regulation of embryonic vascular network formation. The development of the vascular plexus and the accumulation of VE-cadherin at cell-cell junctions were significantly impaired in the RA-GEF-1 knockout allantois and yolk sac. Rap1 activation as visualized by an activation-specific probe was also diminished by RA-GEF-1 knockout. Reduced accumulation of VE-cadherin at cell-cell junctions and defects in blood vessel formation in vitro due to the lack of RA-GEF-1 were suppressed by ectopic expression of constitutively activated Rap1. Overall, these results suggest the involvement of Rap1 downstream of RA-GEF-1 in the regulation of vascular network formation in mouse embryos.
|
| Volume |
387(4)
|
| Pages |
754-9
|
| Published |
2009-10-2
|
| DOI |
10.1016/j.bbrc.2009.07.108
|
| PII |
S0006-291X(09)01469-7
|
| PMID |
19635461
|
| MeSH |
Allantois / blood supply*
Allantois / metabolism
Animals
Antigens, CD / metabolism
Cadherins / metabolism
Cells, Cultured
Female
Guanine Nucleotide Exchange Factors / genetics
Guanine Nucleotide Exchange Factors / physiology*
Mice
Mice, Knockout
Neovascularization, Physiologic / genetics*
Yolk Sac / blood supply*
Yolk Sac / metabolism
rap1 GTP-Binding Proteins / metabolism*
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
CSII-CMV-MCS (RDB04377)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
