RRC ID 1283
Author Ma K, Araki K, Ichwan SJ, Suganuma T, Tamamori-Adachi M, Ikeda MA.
Title E2FBP1/DRIL1, an AT-rich interaction domain-family transcription factor, is regulated by p53.
Journal Mol. Cancer Res.
Abstract E2FBP1/DRIL1 is an AT-rich interaction domain DNA-binding protein and is ubiquitously expressed in various tissues. It has been shown that Bright, the mouse orthologue of E2FBP1/DRIL1, exhibits sequence-specific DNA binding and regulates immunoglobulin transcription. Here we show a novel connection between E2FBP1/DRIL1 and the p53 tumor suppressor, a key regulator of growth arrest or apoptosis in response to cellular stress. We found a putative p53-binding site, which specifically responded to p53, in the second intron of the E2FBP1/DRIL1 gene. E2FBP1/DRIL1was induced by p53 and up-regulated following DNA damage caused by UV radiation or doxorubicin treatment in a manner dependent on endogenous p53. The ectopic expression of E2FBP1/DRIL1 induced growth arrest in U2OS cells expressing normal p53, but not Saos-2 cells lacking p53. These results suggest that E2FBP1/DRIL1 may play a role in growth suppression mediated by p53.
Volume 1(6)
Pages 438-44
Published 2003-4
PMID 12692263
MeSH AT Rich Sequence / genetics* Base Sequence Cell Division Cell Line, Tumor Cyclin D1 / metabolism DNA Damage DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism* Humans Introns / genetics Molecular Sequence Data Oncogenes / genetics* Protein Binding Protein Structure, Tertiary RNA, Messenger / genetics RNA, Messenger / metabolism Trans-Activators* Transcription Factors Transcription, Genetic / genetics Tumor Suppressor Protein p53 / metabolism*
IF 4.597
Times Cited 17
Human and Animal Cells A549