論文 - 詳細
| RRC ID | 1288 |
|---|---|
| 著者 | Adachi Y, Ishii T, Ikeda Y, Hoshino A, Tamura H, Aketagawa J, Tanaka S, Ohno N. |
| タイトル | Characterization of beta-glucan recognition site on C-type lectin, dectin 1. |
| ジャーナル | Infect Immun |
| Abstract |
Dectin 1 is a mammalian cell surface receptor for (1-->3)-beta-d-glucans. Since (1-->3)-beta-d-glucans are commonly present on fungal cell walls, it has been suggested that dectin 1 is important for recognizing fungal invasion. In this study we tried to deduce the amino acid residues in dectin 1 responsible for beta-glucan recognition. HEK293 cells transfected with mouse dectin 1 cDNA could bind to a gel-forming (1-->3)-beta-d-glucan, schizophyllan (SPG). The binding of SPG to a dectin 1 transfectant was inhibited by pretreatment with other beta-glucans having a (1-->3)-beta-d-glucosyl linkage but not by pretreatment with alpha-glucans. Dectin 1 has a carbohydrate recognition domain (CRD) consisting of six cysteine residues that are highly conserved in C-type lectins. We prepared 32 point mutants with mutations in the CRD and analyzed their binding to SPG. Mutations at Trp(221) and His(223) resulted in decreased binding to beta-glucan. Monoclonal antibody 4B2, a dectin- 1 monoclonal antibody which had a blocking effect on the beta-glucan interaction, completely failed to bind the dectin-1 mutant W221A. A mutant with mutations in Trp(221) and His(223) did not have a collaborative effect on Toll-like receptor 2-mediated cellular activation in response to zymosan. These amino acid residues are distinct from residues in other sugar-recognizing peptide sequences of typical C-type lectins. These results suggest that the amino acid sequence W221-I222-H223 is critical for formation of a beta-glucan binding site in the CRD of dectin 1. |
| 巻・号 | 72(7) |
| ページ | 4159-71 |
| 公開日 | 2004-7-1 |
| DOI | 10.1128/IAI.72.7.4159-4171.2004 |
| PII | 72/7/4159 |
| PMID | 15213161 |
| PMC | PMC427417 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal / immunology Binding Sites Biotin / metabolism Glucans / chemistry Glucans / metabolism* Lectins, C-Type / chemistry Lectins, C-Type / metabolism* Membrane Proteins / chemistry Membrane Proteins / genetics Membrane Proteins / immunology Membrane Proteins / metabolism* Mice Molecular Sequence Data Mutation NF-kappa B / metabolism Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Nerve Tissue Proteins / immunology Nerve Tissue Proteins / metabolism* Protein Structure, Tertiary Schizophyllum / genetics Schizophyllum / immunology Schizophyllum / metabolism Sequence Analysis, Protein |
| IF | 3.201 |
| 引用数 | 154 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |