論文 - 詳細
| RRC ID | 1347 |
|---|---|
| 著者 | Nagahama M, Hara Y, Seki A, Yamazoe T, Kawate Y, Shinohara T, Hatsuzawa K, Tani K, Tagaya M. |
| タイトル | NVL2 is a nucleolar AAA-ATPase that interacts with ribosomal protein L5 through its nucleolar localization sequence. |
| ジャーナル | Mol Biol Cell |
| Abstract |
NVL (nuclear VCP-like protein), a member of the AAA-ATPase family, is known to exist in two forms with N-terminal extensions of different lengths in mammalian cells. Here, we show that they are localized differently in the nucleus; NVL2, the major species, is mainly present in the nucleolus, whereas NVL1 is nucleoplasmic. Mutational analysis demonstrated the presence of two nuclear localization signals in NVL2, one of which is shared with NVL1. In addition, a nucleolar localization signal was found to exist in the N-terminal extra region of NVL2. The nucleolar localization signal is critical for interaction with ribosomal protein L5, which was identified as a specific interaction partner of NVL2 on yeast two-hybrid screening. The interaction of NVL2 with L5 is ATP-dependent and likely contributes to the nucleolar translocation of NVL2. The physiological implication of this interaction was suggested by the finding that a dominant negative NVL2 mutant inhibits ribosome biosynthesis, which is known to take place in the nucleolus. |
| 巻・号 | 15(12) |
| ページ | 5712-23 |
| 公開日 | 2004-12-1 |
| DOI | 10.1091/mbc.e04-08-0692 |
| PII | E04-08-0692 |
| PMID | 15469983 |
| PMC | PMC532049 |
| MeSH | ATPases Associated with Diverse Cellular Activities Active Transport, Cell Nucleus Adenosine Triphosphatases / chemistry* Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Cell Line Cell Nucleolus / enzymology* Cell Nucleolus / metabolism Humans Lysine / genetics Lysine / metabolism Molecular Sequence Data Mutation / genetics Nuclear Localization Signals / metabolism* Protein Binding Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / metabolism Protein Subunits / chemistry Protein Subunits / metabolism Ribosomal Proteins / metabolism* Ribosomes / chemistry Ribosomes / metabolism |
| IF | 3.791 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |