論文 - 詳細
| RRC ID | 1374 |
|---|---|
| 著者 | Saitoh M, Nagai K, Nakagawa K, Yamamura T, Yamamoto S, Nishizaki T. |
| タイトル | Adenosine induces apoptosis in the human gastric cancer cells via an intrinsic pathway relevant to activation of AMP-activated protein kinase. |
| ジャーナル | Biochem Pharmacol |
| Abstract |
Extracellular adenosine significantly reduced cell viability in a dose (0.1-20mM)- and treatment time (24-72h)-dependent manner in GT3-TKB cells, a human gastric cancer cell line. Nuclei of cells were reactive to Hoechst 33342, a marker of apoptosis, and an anti-single-stranded DNA. Adenosine-induced GT3-TKB cell death was significantly inhibited by dipyridamole, an inhibitor of adenosine transporter, and 5'-amino-5'-deoxyadenosine, an inhibitor of adenosine kinase, but the effect was not affected by theophylline, a broad inhibitor of adenosine receptors, 8-cyclopentyltheophylline, an inhibitor of A(1) adenosine receptors or 3,7-dimethyl-1-propargylxanthine, an inhibitor of A(2a) adenosine receptors. Adenosine had no effect on mitochondrial membrane potentials. The effect of adenosine on GT3-TKB cell death was not inhibited by a pancaspase inhibitor or inhibitors of caspase-1,-3,-4,-8, and -9. 5-Aminoimidazole-4-carboxamide ribonucleoside (AICAR), an activator of AMP-activated protein kinase (AMPK), significantly reduced GT3-TKB cell viability, but the AICAR action was not reinforced in the presence of adenosine. The results of the present study, thus, suggest that extracellular adenosine induces apoptosis in GT3-TKB cells by its uptake into cells and conversion to AMP followed by activation of AMPK, regardless of caspase activation linked to the mitochondria and the endoplasmic reticulum. |
| 巻・号 | 67(10) |
| ページ | 2005-11 |
| 公開日 | 2004-5-15 |
| DOI | 10.1016/j.bcp.2004.01.020 |
| PII | S0006295204000632 |
| PMID | 15130776 |
| MeSH | Adenine / analogs & derivatives* Adenine / pharmacology Adenosine / pharmacology* Adenylate Kinase / metabolism* Aminoimidazole Carboxamide / analogs & derivatives* Aminoimidazole Carboxamide / pharmacology* Apoptosis* Caspase Inhibitors Dipyridamole / pharmacology Drug Interactions Enzyme Activation / drug effects Humans Membrane Potentials / drug effects Mitochondria / drug effects Mitochondria / physiology Receptors, Purinergic P1 Ribonucleotides / pharmacology* Signal Transduction / drug effects Signal Transduction / physiology Stomach Neoplasms / pathology Tumor Cells, Cultured |
| IF | 4.96 |
| 引用数 | 130 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | GT3TKB(RCB0885) |