論文 - 詳細
| RRC ID | 15304 |
|---|---|
| 著者 | Fujita Y, Tohda H, Giga-Hama Y, Takegawa K. |
| タイトル | Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. |
| ジャーナル | FEMS Yeast Res |
| Abstract |
A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe. |
| 巻・号 | 6(6) |
| ページ | 883-7 |
| 公開日 | 2006-9-1 |
| DOI | 10.1111/j.1567-1364.2006.00093.x |
| PII | FYR093 |
| PMID | 16911510 |
| MeSH | Cadmium / pharmacology Enzyme Activators / pharmacology Ethanol / pharmacology Gene Expression Gene Expression Regulation, Fungal Genes, Reporter Genetic Vectors* Green Fluorescent Proteins / biosynthesis Green Fluorescent Proteins / genetics Heat-Shock Proteins / genetics Hot Temperature Oxidative Stress Promoter Regions, Genetic Recombinant Proteins / biosynthesis* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics |
| IF | 3.193 |
| 引用数 | 9 |
| WOS 分野 | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FYP4216(pAL-SK) FYP4221(pAU-SK) |