RRC ID |
1533
|
著者 |
Kim SW, Her SJ, Kim SY, Shin CS.
|
タイトル |
Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes.
|
巻・号 |
327(3)
|
ページ |
811-9
|
公開日 |
2005-2-18
|
DOI |
10.1016/j.bbrc.2004.12.076
|
PII |
S0006-291X(04)02856-6
|
PMID |
15649418
|
MeSH |
1-Methyl-3-isobutylxanthine / pharmacology
3T3 Cells
Adipocytes / metabolism*
Alkaline Phosphatase / metabolism
Animals
Base Sequence
CCAAT-Enhancer-Binding Protein-alpha / metabolism
Cell Differentiation / physiology*
Cells, Cultured
Dexamethasone / pharmacology
Gene Expression Regulation
Insulin / metabolism
Mice
Osteoblasts / cytology*
PPAR gamma / metabolism
Phenotype
Transcription Factors / genetics
Transcription Factors / physiology*
|
IF |
2.985
|
引用数 |
47
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |