RRC ID 1533
著者 Kim SW, Her SJ, Kim SY, Shin CS.
タイトル Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts.
ジャーナル Biochem Biophys Res Commun
Abstract Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes.
巻・号 327(3)
ページ 811-9
公開日 2005-2-18
DOI 10.1016/j.bbrc.2004.12.076
PII S0006-291X(04)02856-6
PMID 15649418
MeSH 1-Methyl-3-isobutylxanthine / pharmacology 3T3 Cells Adipocytes / metabolism* Alkaline Phosphatase / metabolism Animals Base Sequence CCAAT-Enhancer-Binding Protein-alpha / metabolism Cell Differentiation / physiology* Cells, Cultured Dexamethasone / pharmacology Gene Expression Regulation Insulin / metabolism Mice Osteoblasts / cytology* PPAR gamma / metabolism Phenotype Transcription Factors / genetics Transcription Factors / physiology*
IF 2.985
引用数 47
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)