Reference - Detail
| RRC ID | 1533 |
|---|---|
| Author | Kim SW, Her SJ, Kim SY, Shin CS. |
| Title | Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes. |
| Volume | 327(3) |
| Pages | 811-9 |
| Published | 2005-2-18 |
| DOI | 10.1016/j.bbrc.2004.12.076 |
| PII | S0006-291X(04)02856-6 |
| PMID | 15649418 |
| MeSH | 1-Methyl-3-isobutylxanthine / pharmacology 3T3 Cells Adipocytes / metabolism* Alkaline Phosphatase / metabolism Animals Base Sequence CCAAT-Enhancer-Binding Protein-alpha / metabolism Cell Differentiation / physiology* Cells, Cultured Dexamethasone / pharmacology Gene Expression Regulation Insulin / metabolism Mice Osteoblasts / cytology* PPAR gamma / metabolism Phenotype Transcription Factors / genetics Transcription Factors / physiology* |
| IF | 2.985 |
| Times Cited | 47 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |