RRC ID 15336
Author Yang Y, Jin H, Chen Y, Lin W, Wang C, Chen Z, Han N, Bian H, Zhu M, Wang J.
Title A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana.
Journal New Phytol.
Abstract • A protein encoded by At1g32080 was consistently identified in proteomic studies of Arabidopsis chloroplast envelope membranes, but its function remained unclear. The protein, designated AtLrgB, may have evolved from a gene fusion of lrgA and lrgB. In bacteria, two homologous operons, lrgAB and cidAB, participate in an emerging mechanism to control cell death and lysis. • We aim to characterize AtLrgB using reverse genetics and cell biological and biochemical analysis. • AtLrgB is expressed in leaves, but not in roots. T-DNA insertion mutation of AtLrgB produced plants with interveinal chlorotic and premature necrotic leaves. Overexpression of full-length AtLrgB (or its LrgA and LrgB domains, separately), under the control of CaMV 35S promoter, produced plants exhibiting veinal chlorosis and delayed greening. At the end of light period, the T-DNA mutant had high starch and low sucrose contents in leaves, while the 35S:AtLrgB plants had low starch and high sucrose contents. Metabolite profiling revealed that AtLrgB appeared not to directly transport triose phosphate or hexose phosphates. In yeast cells, AtLrgB could augment nystatin-induced membrane permeability. • Our work indicates that AtLrgB is a new player in chloroplast development, carbon partitioning and leaf senescence, although its molecular mechanism remains to be established.
Volume 193(1)
Pages 81-95
Published 2012-1
DOI 10.1111/j.1469-8137.2011.03867.x
PMID 21916894
MeSH Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / microbiology* Arabidopsis / ultrastructure Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Bacteriolysis* / drug effects Cell Membrane Permeability / drug effects Chloroplast Proteins / chemistry Chloroplast Proteins / metabolism Chloroplasts / drug effects Chloroplasts / metabolism* Chloroplasts / ultrastructure DNA, Bacterial / genetics Evolution, Molecular Gene Expression Regulation, Plant Genes, Plant / genetics Membrane Proteins / chemistry* Membrane Proteins / metabolism* Microbial Viability* / drug effects Mutagenesis, Insertional / genetics Nystatin / pharmacology Operon / genetics Phenotype Phosphates / metabolism Plant Diseases / genetics Plant Diseases / microbiology Protein Structure, Tertiary Protein Transport Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / metabolism Staphylococcus aureus / drug effects Staphylococcus aureus / genetics Starch / metabolism Subcellular Fractions / drug effects Subcellular Fractions / metabolism Sucrose / metabolism
IF 7.433
Times Cited 22
Arabidopsis / Cultured plant cells, genes pda02441