論文 - 詳細
| RRC ID | 1533 |
|---|---|
| 著者 | Kim SW, Her SJ, Kim SY, Shin CS. |
| タイトル | Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Osteoblasts and adipocytes originate from common mesenchymal progenitor cells. We have investigated whether mouse osteoblastic MC3T3-E1 cells can be induced to transdifferentiate into mature adipocytes by the ectopic expression of adipogenic transcription factors, PPARgamma, C/EBPalpha, or both. Retrovirus-mediated overexpression of PPARgamma alone or both PPARgamma and C/EBPalpha resulted in reduced alkaline phosphatase activity and osteoblast-specific gene expression. Moreover, foci of adipocytes were identified in conditions favoring osteoblastic maturation. Upon treatment with insulin, dexamethasone, and IBMX, cells overexpressing PPARgamma alone or both PPARgamma and C/EBPalpha showed marked transdifferentiation to mature adipocytes expressing molecular markers of adipocytes. Cells expressing both PPARgamma and C/EBPalpha showed more robust phenotype of adipocytes than the cells expressing PPARgamma alone. Overexpression of C/EBPalpha alone did not result in adipogenesis. These results suggest that PPARgamma is a key molecular switch for the transdifferentiation to adipocytes whereas C/EBPalpha may differentiate MC3T3-E1 cells into osteoblasts and adipocytes. |
| 巻・号 | 327(3) |
| ページ | 811-9 |
| 公開日 | 2005-2-18 |
| DOI | 10.1016/j.bbrc.2004.12.076 |
| PII | S0006-291X(04)02856-6 |
| PMID | 15649418 |
| MeSH | 1-Methyl-3-isobutylxanthine / pharmacology 3T3 Cells Adipocytes / metabolism* Alkaline Phosphatase / metabolism Animals Base Sequence CCAAT-Enhancer-Binding Protein-alpha / metabolism Cell Differentiation / physiology* Cells, Cultured Dexamethasone / pharmacology Gene Expression Regulation Insulin / metabolism Mice Osteoblasts / cytology* PPAR gamma / metabolism Phenotype Transcription Factors / genetics Transcription Factors / physiology* |
| IF | 2.985 |
| 引用数 | 47 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |