論文 - 詳細
| RRC ID | 15343 |
|---|---|
| 著者 | Miao Y, Li HY, Shen J, Wang J, Jiang L. |
| タイトル | QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells. |
| ジャーナル | J Exp Bot |
| Abstract |
Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures. |
| 巻・号 | 62(14) |
| ページ | 5063-78 |
| 公開日 | 2011-10-1 |
| DOI | 10.1093/jxb/err211 |
| PII | err211 |
| PMID | 21725030 |
| PMC | PMC3193014 |
| MeSH | Amino Acid Sequence Arabidopsis / classification Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Wall / chemistry Cell Wall / enzymology Cell Wall / genetics Cell Wall / metabolism* Cells, Cultured Evolution, Molecular Golgi Apparatus / genetics Methylation Methyltransferases / chemistry Methyltransferases / genetics Methyltransferases / metabolism* Molecular Sequence Data Pectins / biosynthesis Phylogeny Protein Transport Sequence Alignment |
| IF | 5.908 |
| 引用数 | 28 |
| WOS 分野 | PLANT SCIENCES |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst13453 pda00948 pda02310 pda04138 pda04720 pda05472 pda06914 pda09636 pda10373 |