論文 - 詳細
| RRC ID | 15367 |
|---|---|
| 著者 | Hallson G, Hollebakken RE, Li T, Syrzycka M, Kim I, Cotsworth S, Fitzpatrick KA, Sinclair DA, Honda BM. |
| タイトル | dSet1 is the main H3K4 di- and tri-methyltransferase throughout Drosophila development. |
| ジャーナル | Genetics |
| Abstract |
In eukaryotes, the post-translational addition of methyl groups to histone H3 lysine 4 (H3K4) plays key roles in maintenance and establishment of appropriate gene expression patterns and chromatin states. We report here that an essential locus within chromosome 3L centric heterochromatin encodes the previously uncharacterized Drosophila melanogaster ortholog (dSet1, CG40351) of the Set1 H3K4 histone methyltransferase (HMT). Our results suggest that dSet1 acts as a "global" or general H3K4 di- and trimethyl HMT in Drosophila. Levels of H3K4 di- and trimethylation are significantly reduced in dSet1 mutants during late larval and post-larval stages, but not in animals carrying mutations in genes encoding other well-characterized H3K4 HMTs such as trr, trx, and ash1. The latter results suggest that Trr, Trx, and Ash1 may play more specific roles in regulating key cellular targets and pathways and/or act as global H3K4 HMTs earlier in development. In yeast and mammalian cells, the HMT activity of Set1 proteins is mediated through an evolutionarily conserved protein complex known as Complex of Proteins Associated with Set1 (COMPASS). We present biochemical evidence that dSet1 interacts with members of a putative Drosophila COMPASS complex and genetic evidence that these members are functionally required for H3K4 methylation. Taken together, our results suggest that dSet1 is responsible for the bulk of H3K4 di- and trimethylation throughout Drosophila development, thus providing a model system for better understanding the requirements for and functions of these modifications in metazoans. |
| 巻・号 | 190(1) |
| ページ | 91-100 |
| 公開日 | 2012-1-1 |
| DOI | 10.1534/genetics.111.135863 |
| PII | genetics.111.135863 |
| PMID | 22048023 |
| PMC | PMC3249358 |
| MeSH | Amino Acid Sequence Animals Apoptosis Regulatory Proteins / genetics Apoptosis Regulatory Proteins / metabolism Chromosome Mapping DNA, Complementary DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / enzymology* Drosophila melanogaster / genetics Gene Expression Regulation Gene Order Genes, Lethal Genetic Loci Histone Methyltransferases Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism* Histones / genetics Histones / metabolism Methylation Molecular Sequence Data Mutation Sequence Alignment Transcription Factors / genetics Transcription Factors / metabolism Transgenes |
| IF | 4.015 |
| 引用数 | 49 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | Stock(s) of DGRC Kyoto |