論文 - 詳細
| RRC ID | 15371 |
|---|---|
| 著者 | Joti P, Ghosh-Roy A, Ray K. |
| タイトル | Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila. |
| ジャーナル | Sci Rep |
| Abstract |
Stem cell progeny often undergo transit amplifying divisions before differentiation. In Drosophila, a spermatogonial precursor divides four times within an enclosure formed by two somatic-origin cyst cells, before differentiating into spermatocytes. Although germline and cyst cell-intrinsic factors are known to regulate these divisions, the mechanistic details are unclear. Here, we show that loss of dynein-light-chain-1 (DDLC1/LC8) in the cyst cells eliminates bag-of-marbles (bam) expression in spermatogonia, causing gonial cell hyperplasia in Drosophila testis. The phenotype is dominantly enhanced by Dhc64C (cytoplasmic Dynein) and didum (Myosin V) loss-of-function alleles. Loss of DDLC1 or Myosin V in the cyst cells also affects their differentiation. Furthermore, cyst cell-specific loss of ddlc1 disrupts Armadillo, DE-cadherin and Integrin-βPS localizations in the cyst. Together, these results suggest that Dynein and Myosin V activities, and independent DDLC1 functions in the cyst cells organize the somatic microenvironment that regulates spermatogonial proliferation and differentiation. |
| 巻・号 | 1 |
| ページ | 173 |
| 公開日 | 2011-11-30 |
| DOI | 10.1038/srep00173 |
| PII | srep00173 |
| PMID | 22355688 |
| PMC | PMC3240984 |
| MeSH | Alleles Animals Cell Adhesion Cell Differentiation Cell Proliferation Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Dyneins / genetics Dyneins / metabolism* Gene Expression Regulation* Integrin beta1 / metabolism Male Mutation Myosin Type V / genetics Myosin Type V / metabolism Phenotype Spermatogonia / physiology* Testis / physiology rab GTP-Binding Proteins / metabolism |
| IF | 3.998 |
| 引用数 | 17 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | esgGal4/CyO (DGRC Kyoto) UAS-Dhc64CdsRNA/CyO (NIG-Fly) |